Boufis Nazim, Titouh Khayreddine, Khelifi Lakhdar
Laboratoire des Ressources Génétiques et Biotechnologies, Ecole Nationale Supérieure Agronomique (ENSA, ES1603), Hassan Badi Avenue, El-Harrach, Algiers, Algeria.
Division Biotechnologies et Amélioration des Plantes, Institut National de la Recherche Agronomique d'Algérie, BP 200, Hassen Badi Avenue, El-Harrach, Algiers, Algeria.
Methods Mol Biol. 2017;1637:107-117. doi: 10.1007/978-1-4939-7156-5_10.
In vitro plant regeneration via somatic embryogenesis is a powerful tool for rapid, large-scale production of healthy true-to-type plants. This approach is suitable to preserve existing natural genetic variability and propagation of variability generated from genetic improvement programs, including crossing, somaclonal variation, mutagenesis, and somatic hybridization. This chapter outlines a simplified protocol for date palm regeneration via somatic embryogenesis induced in cell suspension cultures. In this protocol, culture medium composition is manipulated, including plant growth regulators and solid (addition of agar) and liquid media to achieve reduction of production cycle of somatic embryogenesis, which increases the multiplication rate of embryogenic callus and improves the quantity and quality of somatic embryos.
通过体细胞胚胎发生进行离体植物再生是快速、大规模生产健康的、与母本基因型一致的植株的有力工具。这种方法适用于保存现有的自然遗传变异性，以及繁殖遗传改良计划（包括杂交、体细胞无性系变异、诱变和体细胞杂交）产生的变异性。本章概述了一种简化的枣椰树再生方案，该方案通过在细胞悬浮培养物中诱导体细胞胚胎发生来实现。在该方案中，对培养基成分进行了调控，包括植物生长调节剂以及固体（添加琼脂）和液体培养基，以缩短体细胞胚胎发生的生产周期，提高胚性愈伤组织的增殖率，并改善体细胞胚的数量和质量。
