Shareef Hussein J
Department of Date Palm Tissue Culture, Date Palm Research Center, University of Basrah, Basrah, Iraq.
Methods Mol Biol. 2017;1637:119-128. doi: 10.1007/978-1-4939-7156-5_11.
Embryogenic suspension cultures of date palm are ideal for mass propagation of somatic embryos; however, the low percentage of germination of somatic embryos (SE) remains an impediment. This chapter focuses on two important physical factors to improve germination of date palm somatic embryos: the use of partial desiccation (3 h) of somatic embryos and the exposure to low temperature (4 °C for 24 h). High germination percentage (41%) is achieved by desiccation for 3 h. Moreover, adding 0.3 g/L activated charcoal (AC) to the liquid medium further improves somatic embryo number and weight as well as the percentage of germination. Moreover, partial desiccation and low temperature exposure tend to increase proline content. This improved protocol for somatic embryo germination is potentially applicable for commercial micropropagation of date palm.
海枣胚性悬浮培养物是体细胞胚大规模繁殖的理想材料；然而，体细胞胚的低萌发率仍然是一个障碍。本章重点介绍提高海枣体细胞胚萌发率的两个重要物理因素：体细胞胚的部分干燥处理（3小时）和低温处理（4℃处理24小时）。通过3小时的干燥处理可实现较高的萌发率（41%）。此外，在液体培养基中添加0.3 g/L活性炭（AC）可进一步提高体细胞胚的数量和重量以及萌发率。此外，部分干燥处理和低温处理往往会增加脯氨酸含量。这种改进的体细胞胚萌发方案可能适用于海枣的商业微繁殖。
