通过衰减全反射傅里叶变换红外光谱法测定二级结构
Secondary Structure Determination by Means of ATR-FTIR Spectroscopy.
作者信息
Srour Batoul, Bruechert Stefan, Andrade Susana L A, Hellwig Petra
机构信息
Laboratoire de Bioelectrochimie et Spectroscopie, UMR 7140, Chimie de la Matière Complexe, Université de Strasbourg, Strasbourg, France.
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104, Freiburg, Germany.
出版信息
Methods Mol Biol. 2017;1635:195-203. doi: 10.1007/978-1-4939-7151-0_10.
Specialized infrared spectroscopic techniques have been developed that allow studying the secondary structure of membrane proteins and the influence of crucial parameters like lipid content and detergent. Here, we focus on an ATR-FTIR spectroscopic study of Af-Amt1 and the influence of LDAO/glycerol on its structural integrity. Our results clearly indicate that infrared spectroscopy can be used to identify the adapted sample conditions.
已经开发出专门的红外光谱技术，可用于研究膜蛋白的二级结构以及脂质含量和去污剂等关键参数的影响。在此，我们重点关注对Af-Amt1的衰减全反射傅里叶变换红外光谱（ATR-FTIR）研究以及月桂胺二氧乙烯（LDAO）/甘油对其结构完整性的影响。我们的结果清楚地表明，红外光谱可用于确定合适的样品条件。
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