Kong Jilie, Yu Shaoning
Department of Chemistry, Fudan University, Shanghai 200433, China.
Acta Biochim Biophys Sin (Shanghai). 2007 Aug;39(8):549-59. doi: 10.1111/j.1745-7270.2007.00320.x.
Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins. It is convenient, non-destructive, requires less sample preparation, and can be used under a wide variety of conditions. This review introduces the recent developments in Fourier transform infrared (FTIR) spectroscopy technique and its applications to protein structural studies. The experimental skills, data analysis, and correlations between the FTIR spectroscopic bands and protein secondary structure components are discussed. The applications of FTIR to the secondary structure analysis, conformational changes, structural dynamics and stability studies of proteins are also discussed.
红外光谱法是分析多肽和蛋白质二级结构最古老且成熟的实验技术之一。它操作便捷、无损、所需样品制备较少，并且能在多种条件下使用。本综述介绍了傅里叶变换红外（FTIR）光谱技术的最新进展及其在蛋白质结构研究中的应用。讨论了实验技巧、数据分析以及FTIR光谱带与蛋白质二级结构成分之间的相关性。还讨论了FTIR在蛋白质二级结构分析、构象变化、结构动力学和稳定性研究中的应用。
