Ortiz Fiorella, Díaz-Barrios Antonio, Lopez-Cabaña Zoraya E, González Gema
School of Chemical Sciences and Engineering, Yachay Tech University, Urcuquí 100119, Ecuador.
Institute of Chemistry of Natural Resources, Universidad de Talca, Talca 3460000, Chile.
Polymers (Basel). 2023 Jul 22;15(14):3121. doi: 10.3390/polym15143121.
Collagen/hydroxyapatite hybrids are promising biomimetic materials that can replace or temporarily substitute bone tissues. The process of biomineralization was carried out through a double diffusion system. The methodological principle consisted in applying an electric field on the incubation medium to promote the opposite migration of ions into collagen membranes to form hydroxyapatite (HA) on the collagen membrane. Two physically separated solutions were used for the incubation medium, one rich in phosphate ions and the other in calcium ions, and their effects were evaluated against the traditional mineralization in Simulated Body Fluid (SBF). Pre-polarization of the organic membranes and the effect of incubation time on the biomineralization process were also assessed by FTIR and Raman spectroscopies.Our results demonstrated that the membrane pre-polarization significantly accelerated the mineralization process on collagen. On the other side, it was found that the application of the electric field influenced the collagen structure and its interactions with the mineral phase. The increment of the mineralization degree enhanced the photoluminescence properties of the collagen/HA materials, while the conductivity and the dielectric constant were reduced. These results might provide a useful approach for future applications in manufacturing biomimetic bone-like materials.
胶原蛋白/羟基磷灰石杂化材料是很有前景的仿生材料，可替代或暂时替代骨组织。生物矿化过程通过双扩散系统进行。该方法的原理是在孵育介质上施加电场，以促进离子向胶原蛋白膜的反向迁移，从而在胶原蛋白膜上形成羟基磷灰石（HA）。孵育介质使用两种物理分离的溶液，一种富含磷酸根离子，另一种富含钙离子，并将它们的效果与模拟体液（SBF）中的传统矿化进行比较评估。还通过傅里叶变换红外光谱（FTIR）和拉曼光谱评估了有机膜的预极化以及孵育时间对生物矿化过程的影响。我们的结果表明，膜预极化显著加速了胶原蛋白上的矿化过程。另一方面，发现施加电场会影响胶原蛋白结构及其与矿相的相互作用。矿化程度的增加增强了胶原蛋白/HA材料的光致发光性能，同时降低了电导率和介电常数。这些结果可能为未来制造仿生骨样材料的应用提供一种有用的方法。
