Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
J Pharm Biomed Anal. 2018 Jan 5;147:400-416. doi: 10.1016/j.jpba.2017.06.071. Epub 2017 Jul 3.
This review article is the first in the series providing an overview of currently used antiviral drugs and presenting contemporary approaches to their analysis. Large number of available antivirals and their structural variability makes this task very challenging. Trying to cover this topic comprehensively while maintaining reasonable size of the article, the review is presented in two parts. For the purpose of the overall review, antivirals were divided into four groups: (i) antivirals against herpes viruses, (ii) antivirals against respiratory viruses, (iii) antivirals against hepatitis viruses, and (iv) antivirals against HIV. Part one is devoted to the groups (i) and (ii) and also concerns the key features of the bioanalytical method. The mechanisms of action of antivirals against respiratory and herpes viruses and their use in clinical practice are briefly outlined, and the analytical methods for selected representatives of each class are described in more detail. The methods developed for the determination of drugs from these classes mostly include conventional procedures. In contrast, current trends such as UHPLC are used rarely and proper method validation based on requirements of bioanalytical guidelines can be often considered insufficient.
这篇综述文章是系列文章的第一篇，旨在概述目前使用的抗病毒药物，并介绍其分析的现代方法。大量现有的抗病毒药物及其结构的可变性使得这项任务极具挑战性。为了全面涵盖这一主题，同时保持文章的合理篇幅，本综述分为两部分。为了全面综述的目的，将抗病毒药物分为四组：（i）抗疱疹病毒药物，（ii）抗呼吸道病毒药物，（iii）抗肝炎病毒药物，和（iv）抗 HIV 药物。第一部分专门讨论了第（i）组和第（ii）组，并涉及到生物分析方法的关键特征。简要概述了抗呼吸道和疱疹病毒药物的作用机制及其在临床实践中的应用，并详细描述了每类药物的代表性药物的分析方法。这些类别的药物的测定方法主要包括常规程序。相比之下，超高效液相色谱法等当前趋势很少使用，并且根据生物分析指南的要求进行适当的方法验证通常被认为是不够的。
