Ushigome S, Takakuwa T, Takagi M, Koizumi H, Morikubo M
Acta Pathol Jpn. 1986 Jul;36(7):963-71. doi: 10.1111/j.1440-1827.1986.tb00207.x.
Cases of proliferative myositis and fasciitis were studied immunohistochemically and ultrastructurally for further understanding of the nature of ganglion cell-like giant cells. Blood coagulation factor XIIIa, fibronectin, myoglobin, myosin, CPK MM, and alpha-1-antichymotrypsin were detected in three cases of proliferative myositis and two cases of proliferative fasciitis by the avidin-biotin-peroxidase complex method. Factor XIIIa (a fibrin-stabilizing factor) and fibronectin were strongly positive in the giant cells, but not in striated muscle fibers. A small quantity of myosin was demonstrated in the giant cells, but myoglobin and CPK MM were never demonstrated in these cells. No alpha-1-antichymotrypsin was demonstrated in the giant cells. One case of proliferative myositis showed ultrastructural features suggestive of fibroblast rather than muscle cell or histiocytic origin. Strongly positive factor XIIIa in the giant cells is suggestive of the fact that they are active fibroblasts.
为进一步了解神经节细胞样巨细胞的本质，对增生性肌炎和筋膜炎病例进行了免疫组织化学和超微结构研究。采用抗生物素蛋白-生物素-过氧化物酶复合物法，在3例增生性肌炎和2例增生性筋膜炎中检测到凝血因子XIIIa、纤连蛋白、肌红蛋白、肌球蛋白、肌酸磷酸激酶MM和α1抗胰凝乳蛋白酶。因子XIIIa（一种纤维蛋白稳定因子）和纤连蛋白在巨细胞中呈强阳性，但在横纹肌纤维中不呈阳性。在巨细胞中证实有少量肌球蛋白，但在这些细胞中从未证实有肌红蛋白和肌酸磷酸激酶MM。在巨细胞中未证实有α1抗胰凝乳蛋白酶。1例增生性肌炎显示出超微结构特征，提示其起源于成纤维细胞而非肌肉细胞或组织细胞。巨细胞中因子XIIIa呈强阳性提示它们是活跃的成纤维细胞。
