Shokri Azar, Akhtari Javad, Keighobadi Masoud, Fakhar Mahdi, Teshnizi Saeed Hosseini, Emami Saeed, Sadjjadian Sajede
Students Research Committee, Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Molecular and Cell Biology Research Center, Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Immunogenetic Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Asian Pac J Trop Med. 2017 Jun;10(6):544-548. doi: 10.1016/j.apjtm.2016.09.014. Epub 2017 Jun 19.
To evaluate the effect of doxorubicin and its pegylated liposomal formulation (Doxil, Caelyx) on in vitro susceptibility of promastigote and amastigote stages of Leishmania major.
Throughout in vitro assays the IC was calculated in the promastigotes and amastigotes forms in J774 macrophage cell line. Also as cytotoxicity in J774 cell line macrophages.
Doxorubicin and Doxil showed the same activity against promastigote form with IC values of 10.49 μg/mL and 9.63 μg/mL, respectively. Similarly, the amastigote stage was susceptible at concentration of at least 1 μg/mL when compared to positive control (P < 0.0001). Also, cytotoxicity assay against macrophage revealed no toxicity on the host cells at IC concentrations.
Our findings demonstrated the efficacy of both doxorubicin and its pegylated liposomal formulation on L. major at low concentrations. Further researches are needed for evaluating the safety of drugs in animal model particularly as topical formulation.
评估阿霉素及其聚乙二醇化脂质体制剂（多柔比星脂质体，商品名：多美素、凯素灵）对硕大利什曼原虫前鞭毛体和无鞭毛体阶段体外易感性的影响。
在整个体外试验中，计算J774巨噬细胞系中前鞭毛体和无鞭毛体形式的半数抑制浓度（IC）。同时也计算其对J774细胞系巨噬细胞的细胞毒性。
阿霉素和多柔比星脂质体对前鞭毛体形式表现出相同的活性，IC值分别为10.49μg/mL和9.63μg/mL。同样，与阳性对照相比，无鞭毛体阶段在至少1μg/mL的浓度下敏感（P<0.0001）。此外，针对巨噬细胞的细胞毒性试验表明，在IC浓度下对宿主细胞无毒性。
我们的研究结果表明，阿霉素及其聚乙二醇化脂质体制剂在低浓度下对硕大利什曼原虫均有效。需要进一步研究以评估药物在动物模型中的安全性，特别是作为局部制剂时。
