Artika I Made, Julistiono Heddy, Bermawie Nurliani, Riyanti Eny Ida, Hasan Akhmad Endang Zainal
Department of Biochemistry, Faculty of Mathematics and Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia.
Department of Biochemistry, Faculty of Mathematics and Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia.
Asian Pac J Trop Med. 2017 Jun;10(6):566-571. doi: 10.1016/j.apjtm.2017.06.004. Epub 2017 Jun 20.
To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.).
Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. At an extract concentration of 100 μg/mL, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL and 400 μg/mL.
Results showed that isolate Sir-G5 had the highest anticancer activity with an IC of 19.20 μg/mL. The best isolates were screened again using a normal cell (Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phomopsis sp.
The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.
分析从番荔枝叶（番荔枝）中分离出的内生真菌的乙酸乙酯提取物的抗癌活性。
采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法，通过观察真菌提取物对MCF-7（密歇根癌症基金会-7）细胞的体外毒性来测定其抗癌活性。在提取物浓度为100μg/mL时，12株分离物中有4株对癌细胞生长表现出高活性。然后选择这4株分离物进行进一步的半数抑制浓度（IC）测定，通过测量在提取物浓度为25μg/mL、50μg/mL、100μg/mL、200μg/mL和400μg/mL时对癌细胞增殖的抑制作用。
结果表明，分离物Sir-G5具有最高的抗癌活性，半数抑制浓度为19.20μg/mL。再次使用正常细胞（Chang细胞）对最佳分离物进行筛选，以确定其对正常细胞的毒性。结果表明，提取物不影响正常细胞的增殖。分子鉴定表明，真菌分离物Sir-G5与拟茎点霉属有密切关系。
从番荔枝叶中分离出的内生真菌有潜力用作抗癌剂的来源。
