Poulsen Louise Ladefoged, Thøfner Ida, Bisgaard Magne, Christensen Jens Peter, Olsen Rikke Heidemann, Christensen Henrik
University of Copenhagen, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, Stigbøjlen 4, DK, 1870 Frederiksberg, Denmark.
University of Copenhagen, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, Stigbøjlen 4, DK, 1870 Frederiksberg, Denmark.
Vet Microbiol. 2017 Aug;207:13-18. doi: 10.1016/j.vetmic.2017.05.029. Epub 2017 Jun 1.
Escherichia coli is of major importance in industrial broiler production as the main cause of salpingitis and peritonitis in broiler breeders. Furthermore E. coli is the most common cause of first week mortality in broiler chickens. The aim of the present study was to investigate the transmission of E. coli, isolated from broiler breeders with salpingitis, to the progeny and the possibility of subsequent first week mortality. Four parent flocks were followed during the whole production period (20-60 weeks) by post mortem and bacteriological examination of randomly selected dead birds. Newly hatched chickens from each flock were swabbed in the cloaca on four occasions (parent age 30, 40, 50, 60 weeks) and E. coli was isolated. Causes of first week mortality were determined pathologically and bacteriologically. E. coli isolates from parents, newly hatched chickens and first week mortality were selected for Pulsed-Field-Gel-Electrophoresis (PFGE) and Multi-Locus-Sequence-Typing (MLST) to determine their clonal relationships. E. coli was the main cause of both salpingitis in parents and first week mortality in broilers, and E. coli dominated the bacterial flora of the cloaca of newly hatched chickens. PFGE of E. coli showed identical band patterns in isolates from the three different sources indicating a transmission of E. coli from parent birds to chickens. In conclusion, E. coli isolated from salpingitis in broiler parents were found to be transmitted to broilers in which some sequence types contributed to the first week mortality.
大肠杆菌在工业肉鸡生产中至关重要，是肉种鸡输卵管炎和腹膜炎的主要病因。此外，大肠杆菌是肉鸡第一周龄死亡率的最常见原因。本研究的目的是调查从患有输卵管炎的肉种鸡中分离出的大肠杆菌向后代的传播情况以及随后第一周龄死亡率的可能性。在整个生产期（20 - 60周）内，通过对随机选择的死禽进行尸检和细菌学检查，跟踪了四个亲本鸡群。在四个时间点（亲本鸡龄30、40、50、60周）对每个鸡群新孵化的雏鸡进行泄殖腔拭子采样，并分离出大肠杆菌。通过病理学和细菌学方法确定第一周龄死亡率的原因。选择来自亲本、新孵化雏鸡和第一周龄死亡雏鸡的大肠杆菌分离株进行脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST），以确定它们的克隆关系。大肠杆菌是亲本输卵管炎和肉鸡第一周龄死亡率的主要原因，并且大肠杆菌在新孵化雏鸡的泄殖腔菌群中占主导地位。大肠杆菌的PFGE显示，来自三种不同来源的分离株具有相同的条带模式，表明大肠杆菌从亲本传播到雏鸡。总之，从肉种鸡输卵管炎中分离出的大肠杆菌被发现可传播给肉鸡，其中一些序列类型导致了第一周龄死亡率。
