Poulsen Louise Ladefoged, Bisgaard Magne, Christensen Henrik
Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark.
Bisgaard Consulting, 4130 Viby Sjælland, Denmark.
Antibiotics (Basel). 2023 Feb 7;12(2):344. doi: 10.3390/antibiotics12020344.
Avian pathogenic (APEC) are important bacteria in broiler production in terms of economy, welfare, and use of antibiotics. During a previous outbreak of APEC in the Nordic countries, it was suggested that the pathogenic clones of causing the outbreak originated from grandparent stock and were transmitted to the offspring, causing increased first week mortality. This study investigated whether the pathogenic potential of at the parent and broiler level differs in relation to pathogenic potential described by the level of virulence-associated genes and pattern of antimicrobial resistance. The hypothesis was that, due to higher biosecurity at the parent level, the population will show a lower level of antimicrobial resistance and carry fewer virulence-associated genes, as a result of fewer infections observed. From four parent flocks and eight broiler flocks, 715 were isolated from cloacal swabs of newly hatched chickens (Ross 308). The isolated were characterized by eight virulence-associated genes and phenotypic resistance against six antimicrobials. It was found that the prevalence of virulence-associated genes and phenotypic antimicrobial resistance varied significantly between flocks, and the virulence-associated genes and and resistance against ampicillin were significantly more prevalent in breeder flocks compared to broiler flocks.
禽致病性大肠杆菌（APEC）在肉鸡生产中，在经济、福利以及抗生素使用方面都是重要的细菌。在北欧国家之前爆发的一次APEC疫情中，有人提出引发疫情的致病性克隆菌株源自祖代种鸡，并传播给了后代，导致第一周死亡率上升。本研究调查了在亲本和肉鸡层面，大肠杆菌的致病潜力与由毒力相关基因水平和抗菌药物耐药模式所描述的致病潜力相比是否存在差异。假设是，由于亲本层面的生物安全措施更高，大肠杆菌群体将表现出较低水平的抗菌药物耐药性，并且携带的毒力相关基因更少，这是因为观察到的感染较少。从四个亲本鸡群和八个肉鸡鸡群中，从新孵化鸡（罗斯308）的泄殖腔拭子中分离出715株大肠杆菌。对分离出的大肠杆菌进行了八个毒力相关基因以及对六种抗菌药物的表型耐药性鉴定。结果发现，毒力相关基因和表型抗菌药物耐药性的流行率在不同鸡群之间存在显著差异，与肉鸡鸡群相比，毒力相关基因fimH和iroN以及对氨苄西林的耐药性在种鸡群中显著更为普遍。
