Shiosaka S, Kiyama H, Wanaka A, Tohyama M
Brain Res. 1986 Sep 24;382(2):399-403. doi: 10.1016/0006-8993(86)91352-1.
Antiserum against the glutamate was developed using colloidal gold as a carrier for the antigen. Glutamate was bound directly to colloidal gold without using cross-linking reagents such as glutaraldehyde, formaldehyde, or carbodiimide. Repeated injection of the colloidal gold-glutamate complex into the rabbit yielded a specific, high titre antiserum. Specificity was verified by radioimmunoassay and histochemically with absorption controls. The antiserum immunohistochemically detected numerous glutamate-containing neurons in the rat brain.
以胶体金作为抗原载体研制出了抗谷氨酸抗血清。谷氨酸直接与胶体金结合，无需使用诸如戊二醛、甲醛或碳二亚胺等交联试剂。将胶体金 - 谷氨酸复合物反复注射到兔子体内，产生了特异性的高滴度抗血清。通过放射免疫测定以及用吸收对照进行组织化学检测来验证特异性。该抗血清通过免疫组织化学方法在大鼠脑中检测到了众多含谷氨酸的神经元。
