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趋化因子（CCL3、CCL4 和 CCL5）抑制单核细胞中 ATP 诱导的 IL-1 释放。

Chemokines (CCL3, CCL4, and CCL5) Inhibit ATP-Induced Release of IL-1 by Monocytic Cells.

机构信息

Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus-Liebig-University Giessen, Giessen, Germany.

Department of Biochemistry, Faculty of Medicine, Universities of Giessen and Marburg Lung Center, Giessen, Germany.

出版信息

Mediators Inflamm. 2017;2017:1434872. doi: 10.1155/2017/1434872. Epub 2017 Jul 5.

DOI:10.1155/2017/1434872
PMID:28757683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5516742/
Abstract

Chemokines and ATP are among the mediators of inflammatory sites that can enter the circulation via damaged blood vessels. The main function of chemokines is leukocyte mobilization, and ATP typically triggers inflammasome assembly. IL-1, a potent inflammasome-dependent cytokine of innate immunity, is essential for pathogen defense. However, excessive IL-1 may cause life-threatening systemic inflammation. Here, we hypothesize that chemokines control ATP-dependent secretion of monocytic IL-1. Lipopolysaccharide-primed human monocytic U937 cells were stimulated with the P2X7 agonist BzATP for 30 min to induce IL-1 release. CCL3, CCL4, and CCL5 dose dependently inhibited BzATP-stimulated release of IL-1, whereas CXCL16 was ineffective. The effect of CCL3 was confirmed for primary mononuclear leukocytes. It was blunted after silencing CCR1 or calcium-independent phospholipase A2 (iPLA2) by siRNA and was sensitive to antagonists of nicotinic acetylcholine receptors containing subunits 7 and 9. U937 cells secreted small factors in response to CCL3 that mediated the inhibition of IL-1 release. We suggest that CCL chemokines inhibit ATP-induced release of IL-1 from U937 cells by a triple-membrane-passing mechanism involving CCR, iPLA2, release of small mediators, and nicotinic acetylcholine receptor subunits 7 and 9. We speculate that whenever chemokines and ATP enter the circulation concomitantly, systemic release of IL-1 is minimized.

摘要

趋化因子和 ATP 是炎症部位的介质之一，它们可以通过受损的血管进入循环系统。趋化因子的主要功能是动员白细胞，而 ATP 通常会引发炎症小体的组装。IL-1 是先天免疫中一种有效的炎症小体依赖性细胞因子，对病原体防御至关重要。然而，过量的 IL-1 可能会导致危及生命的全身炎症。在这里，我们假设趋化因子控制着单核细胞 IL-1 的 ATP 依赖性分泌。用 P2X7 激动剂 BzATP 刺激脂多糖预刺激的人单核细胞 U937 细胞 30 分钟，以诱导 IL-1 释放。CCL3、CCL4 和 CCL5 呈剂量依赖性抑制 BzATP 刺激的 IL-1 释放，而 CXCL16 则无效。CCL3 的作用在原代单核细胞中得到了证实。用 siRNA 沉默 CCR1 或钙非依赖性磷脂酶 A2（iPLA2）后，其作用减弱，并且对含有亚基 7 和 9 的烟碱型乙酰胆碱受体拮抗剂敏感。U937 细胞分泌小因子以响应 CCL3，介导 IL-1 释放的抑制。我们认为，CCL 趋化因子通过涉及 CCR、iPLA2、小介质释放和烟碱型乙酰胆碱受体亚基 7 和 9 的三重膜传递机制抑制 U937 细胞中 ATP 诱导的 IL-1 释放。我们推测，只要趋化因子和 ATP 同时进入循环系统，全身释放的 IL-1 就会最小化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/21dbcae46486/MI2017-1434872.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/fdc01cc34283/MI2017-1434872.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/f8e956c0b610/MI2017-1434872.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/7acc39c77144/MI2017-1434872.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/a67d910fe657/MI2017-1434872.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/6f79571be68e/MI2017-1434872.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/96d3125eb044/MI2017-1434872.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/21dbcae46486/MI2017-1434872.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/fdc01cc34283/MI2017-1434872.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/f8e956c0b610/MI2017-1434872.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/7acc39c77144/MI2017-1434872.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/a67d910fe657/MI2017-1434872.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/6f79571be68e/MI2017-1434872.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/96d3125eb044/MI2017-1434872.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f3c/5516742/21dbcae46486/MI2017-1434872.007.jpg

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2
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3
Purinergic regulation of the immune system.
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4
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Sci Rep. 2022 Jul 9;12(1):11714. doi: 10.1038/s41598-022-15668-8.
5
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JCI Insight. 2022 Aug 8;7(15):e156320. doi: 10.1172/jci.insight.156320.
6
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4
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Antioxid Redox Signal. 2015 Dec 10;23(17):1370-87. doi: 10.1089/ars.2015.6398.
5
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J Immunol. 2015 Sep 1;195(5):2325-34. doi: 10.4049/jimmunol.1400974. Epub 2015 Jul 22.
6
Calcium-independent phospholipases A2 and their roles in biological processes and diseases.不依赖钙的磷脂酶A2及其在生物过程和疾病中的作用。
J Lipid Res. 2015 Sep;56(9):1643-68. doi: 10.1194/jlr.R058701. Epub 2015 May 28.
7
The systemic immune response to trauma: an overview of pathophysiology and treatment.创伤的全身免疫反应：病理生理学与治疗概述
Lancet. 2014 Oct 18;384(9952):1455-65. doi: 10.1016/S0140-6736(14)60687-5. Epub 2014 Oct 17.
8
Mechanisms and functions of inflammasomes.炎性小体的作用机制和功能。
Cell. 2014 May 22;157(5):1013-22. doi: 10.1016/j.cell.2014.04.007.
9
Immunoinflammatory response in critically ill patients: severe sepsis and/or trauma.危重症患者的免疫炎症反应：严重脓毒症和/或创伤。
Mediators Inflamm. 2013;2013:362793. doi: 10.1155/2013/362793. Epub 2013 Nov 24.
10
Inflammasomes and host defenses against bacterial infections.炎性小体与宿主防御细菌感染
Curr Opin Microbiol. 2013 Feb;16(1):23-31. doi: 10.1016/j.mib.2012.11.008. Epub 2013 Jan 11.