Badstuebner Kathrin, Gimsa Ulrike, Weber Immo, Tuchscherer Armin, Gimsa Jan
Department of Neurology, University of Rostock, Rostock, Germany.
Institute of Behavioural Physiology, Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany.
Parkinsons Dis. 2017;2017:5693589. doi: 10.1155/2017/5693589. Epub 2017 Jul 3.
Although the clinical use of deep brain stimulation (DBS) is increasing, its basic mechanisms of action are still poorly understood. Platinum/iridium electrodes were inserted into the subthalamic nucleus of rats with unilateral 6-OHDA-induced lesions of the medial forebrain bundle. Six behavioral parameters were compared with respect to their potential to detect DBS effects. Locomotor function was quantified by (i) apomorphine-induced rotation, (ii) initiation time, (iii) the number of adjusting steps in the stepping test, and (iv) the total migration distance in the open field test. Sensorimotor neglect and anxiety were quantified by (v) the retrieval bias in the corridor test and (vi) the ratio of migration distance in the center versus in the periphery in the open field test, respectively. In our setup, unipolar stimulation was found to be more efficient than bipolar stimulation for achieving beneficial long-term DBS effects. Performance in the apomorphine-induced rotation test showed no improvement after 6 weeks. DBS reduced the initiation time of the contralateral paw in the stepping test after 3 weeks of DBS followed by 3 weeks without DBS. Similarly, sensorimotor neglect was improved. The latter two parameters were found to be most appropriate for judging therapeutic DBS effects.
尽管脑深部电刺激（DBS）的临床应用正在增加，但其基本作用机制仍知之甚少。将铂/铱电极插入单侧6-羟基多巴胺诱导内侧前脑束损伤的大鼠丘脑底核。比较了六个行为参数检测DBS效应的潜力。运动功能通过以下方式量化：（i）阿扑吗啡诱导的旋转，（ii）起始时间，（iii）步测中的调整步数，以及（iv）旷场试验中的总迁移距离。感觉运动忽视和焦虑分别通过（v）走廊试验中的检索偏差和（vi）旷场试验中中央与周边迁移距离的比率来量化。在我们的实验装置中，发现单极刺激在实现有益的长期DBS效应方面比双极刺激更有效。阿扑吗啡诱导的旋转试验中的表现在6周后没有改善。在进行3周DBS后再停止3周DBS，DBS减少了步测中对侧爪的起始时间。同样，感觉运动忽视也得到了改善。发现后两个参数最适合判断治疗性DBS效应。
