Andleeb Saiqa, Naseer Anum, Ali Shaukat, Mustafa Rozina Ghulam, Zafar Atiya, Shafique Irsa, Ismail Muhammad, Saleem Muhammad, Mansoor Qaiser
Department of Zoology, Microbial Biotechnology Laboratory, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan 13100, Pakistan.
Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
Infect Disord Drug Targets. 2018;18(2):164-176. doi: 10.2174/1871526517666170728130836.
Human infectious diseases are caused by various pathogens including bacteria, fungi, viruses, parasites, and protozoans. These infectious agents are controlled by using synthetic drugs as well as natural sources.
The aim of current study was to evaluate the antibacterial effect of Rumex hastatus against clinical bacterial pathogens.
In current research antibacterial effect of Rumex hastatus was analyzed against seven clinical pathogenic bacteria such as Escherichia coli, Serratia marcescens, Streptococcus pyogenes, Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa through agar well diffusion method. The boiled extract was used for the phytochemical screening, antioxidant potential, thin layer chromatography, bio-autography, and spot screening. Genomic DNA was extracted to find the DNA protection effect of R. hastatus.
Antibacterial results showed that diethyl ether extract has the maximum inhibition of S. pyogenes (9.66 ± 0.57 mm). Acetone and diethyl ether extracts showed moderate inhibition of K. pneumoniae (6.33 ± 1.52 mm and 5.66 ±1.15 mm) and S. aureus (6.33 ± 1.52 mm and 5.66 ± 0.57 mm). Similarly, chloroform extract indicated moderate inhibition of S. pyogenes (5.66 ± 1.15 mm). Ethanol extract had low or even no effect on the growth of bacteria. Genomic DNA extraction also encouraged the antibacterial effect of R. hastatus. Various phytochemical constituents such as ketoses, oligosaccharides, amino acids, amines, sugars, flavonoids, and antioxidant constituents were detected. TLC-Bioautography and spot screening results revealed the potential use of R. hustatus as an antibacterial agent.
It was concluded that most of the tested fractions appeared as an important source for the discovery of new antimicrobial drugs.
人类传染病由多种病原体引起，包括细菌、真菌、病毒、寄生虫和原生动物。这些传染源可通过使用合成药物以及天然来源进行控制。
本研究旨在评估戟叶酸模对临床细菌病原体的抗菌作用。
在本研究中，通过琼脂扩散法分析了戟叶酸模对七种临床致病细菌的抗菌作用，这些细菌包括大肠杆菌、粘质沙雷氏菌、化脓性链球菌、表皮葡萄球菌、金黄色葡萄球菌、肺炎克雷伯菌和铜绿假单胞菌。将煮沸的提取物用于植物化学筛选、抗氧化潜力、薄层色谱、生物自显影和斑点筛选。提取基因组DNA以研究戟叶酸模的DNA保护作用。
抗菌结果表明，乙醚提取物对化脓性链球菌的抑制作用最大（9.66±0.57毫米）。丙酮和乙醚提取物对肺炎克雷伯菌（6.33±1.52毫米和5.66±1.15毫米）和金黄色葡萄球菌（6.33±1.52毫米和5.66±0.57毫米）有中度抑制作用。同样，氯仿提取物对化脓性链球菌有中度抑制作用（5.66±1.15毫米）。乙醇提取物对细菌生长的影响较小甚至没有影响。基因组DNA提取也增强了戟叶酸模的抗菌作用。检测到了各种植物化学成分，如酮糖、寡糖、氨基酸、胺、糖、黄酮类化合物和抗氧化成分。薄层色谱-生物自显影和斑点筛选结果表明戟叶酸模有作为抗菌剂的潜在用途。
得出的结论是，大多数测试馏分是发现新型抗菌药物的重要来源。
