Applied Microbiology Research Center, System Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Sheikh Bahaei Street, Molla Sadra Street, Vanak Sq., Tehran 984359-44711, Iran.
Applied Microbiology Research Center, System Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Sheikh Bahaei Street, Molla Sadra Street, Vanak Sq., Tehran 984359-44711, Iran.
Microb Pathog. 2018 Jan;114:204-208. doi: 10.1016/j.micpath.2017.12.002. Epub 2017 Dec 5.
The aim of this study was to investigate the antibacterial activities and phytochemical analysis of extracts against the growth of some pathogenic strain causing poisoning and infection (Staphylococcus aureus, Streptococcus pyogenes, Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli and Shigella flexneri). Makhlaseh components were identified via gas chromatography/mass spectrometry (GC/MS). Total phenolic content (TPC), alkaloids, tannins and saponins were determined. Antioxidant activity was determined calorimetrically for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Antimicrobial effect of extracts was evaluated by five methods, pour plate, well diffusion, disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). Camphor was the major compound of Makhlaseh. The TPC of aqueous and ethanolic Makhlaseh extracts was equal to 79.45 ± 1.15 and 115.26 ± 1.23 μg GAE/mg, respectively. The antioxidant activity (IC) test of aqueous and ethanolic Makhlaseh extracts showed 315.50 ± 1.12 and 118.35 ± 1.08 μg/ml, respectively. MIC of the aqueous extract of Makhlaseh for Enterobacter aerogenes, Escherichia coli, Shigella flexneri, Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes were 32, 32, 16, 16, 8 and 8 mg/ml, respectively, and the MIC of the ethanolic extract were 16, 16, 16, 8, 4, and 4 mg/ml, respectively. The MBCs of the Makhlaseh extracts varied from 4 mg/ml to 128 mg/ml. Increasing concentration of Makhlaseh extracts had a significant effect (p ≤ 0.05) on inhibition zone diameter. In conclusion, using Makhlaseh extracts as a natural antibacterial composite in vitro have significant antibacterial ability over the studied strains.
本研究旨在研究提取物对引起中毒和感染的一些病原菌（金黄色葡萄球菌、化脓性链球菌、表皮葡萄球菌、产气肠杆菌、大肠杆菌和福氏志贺菌）的生长的抗菌活性和植物化学分析。通过气相色谱/质谱联用仪（GC/MS）鉴定 Makhlaseh 成分。测定总酚含量（TPC）、生物碱、单宁和皂苷。通过 2,2-二苯基-1-苦基肼（DPPH）清除活性的比色法测定抗氧化活性。通过五种方法评估提取物的抗菌效果，即倾注平板法、孔扩散法、圆盘扩散法、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。莰烯是 Makhlaseh 的主要成分。水提和醇提 Makhlaseh 的 TPC 分别为 79.45±1.15 和 115.26±1.23μg GAE/mg。水提和醇提 Makhlaseh 提取物的抗氧化活性（IC）试验分别为 315.50±1.12 和 118.35±1.08μg/ml。水提 Makhlaseh 对产气肠杆菌、大肠杆菌、福氏志贺菌、金黄色葡萄球菌、表皮葡萄球菌和化脓性链球菌的 MIC 分别为 32、32、16、16、8 和 8mg/ml，醇提 Makhlaseh 的 MIC 分别为 16、16、16、8、4 和 4mg/ml。Makhlaseh 提取物的 MBC 从 4mg/ml 到 128mg/ml 不等。Makhlaseh 提取物浓度的增加对抑菌圈直径有显著影响（p≤0.05）。综上所述，体外使用 Makhlaseh 提取物作为天然抗菌复合物对研究菌株具有显著的抗菌能力。
