Boontham Wanatchaporn, Limtong Savitree, Rosa Carlos A, Lopes Mariana R, Vital Marcos J S, Srisuk Nantana
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Center for Advanced Studies in Tropical Natural Resources, National Research University Kasetsart University, Thailand.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2569-2573. doi: 10.1099/ijsem.0.001970. Epub 2017 Jul 31.
Two yeast strains, DMKU-WBBC14 and UFMG-CM-Y3283, were isolated from soil in Samutprakarn province in the central part of Thailand and from rotting wood in an Amazonian forest site in the Roraima State in Brazil, respectively. The two strains showed identical sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene and different sequences by three nucleotide substitutions of the internal transcribed spacer (ITS) region. Therefore, these two strains represented a single species which was most closely related to Cyberlindnera mengyuniae CBS 10845T. The nucleotide sequence differences between the two strains of the novel species and the type strain Cyberlindnera mengyuniae CBS 10845T were 10 substitutions in the D1/D2 region of the LSU rRNA gene and 46 substitutions in the ITS region. DMKU-WBBC14 and UFMG-CM-Y3283 differed in growth temperature profiles. Moreover, they also exhibited different carbon assimilation profiles and growth temperature profiles from the type strain of Cyberlindnera mengyuniae, CBS 10845T. The name Cyberlindnera tropicalis f.a., sp. nov. is proposed. The type strain is DMKU-WBBC14T (=CBS 14558T=TBRC 6562T). The Mycobank number is MB 819722.
两株酵母菌株，DMKU-WBBC14和UFMG-CM-Y3283，分别从泰国中部巴吞他尼府的土壤以及巴西罗赖马州一个亚马逊森林地区的腐烂木材中分离得到。这两株菌株的大亚基（LSU）rRNA基因的D1/D2结构域序列相同，但内部转录间隔区（ITS）区域有三个核苷酸替换导致序列不同。因此，这两株菌株代表了一个与蒙氏嗜林酵母CBS 10845T关系最为密切的单一物种。该新物种的两株菌株与模式菌株蒙氏嗜林酵母CBS 10845T在LSU rRNA基因的D1/D2区域有10个替换，在ITS区域有46个替换。DMKU-WBBC14和UFMG-CM-Y3283在生长温度曲线方面存在差异。此外，它们与蒙氏嗜林酵母的模式菌株CBS 10845T在碳同化曲线和生长温度曲线上也表现不同。现提出热带嗜林酵母f.a.，新种。模式菌株为DMKU-WBBC14T（=CBS 14558T=TBRC 656/T）。国际真菌索引编号为MB 819722。
