Polburee Pirapan, Lertwattanasakul Noppon, Limtong Pitayakon, Groenewald Marizeth, Limtong Savitree
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Rattanakosin College for Sustainable Energy and Environment (RCSEE), Rajamangala University of Technology Rattanakosin, Nakhon Pathom, Thailand.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2377-2382. doi: 10.1099/ijsem.0.001961. Epub 2017 Jul 21.
Strain DMKU-PS11(1)T was isolated from peat in a swamp forest in Thailand. DNA sequence analysis showed that it belonged to a novel species that was most closely related to Nakazawaea laoshanensis. However, it differed from the type strain of N. laoshanensis (NRRL Y-63634T) by 2.3 % nucleotide substitutions in the D1/D2 region of the large subunit (LSU) rRNA gene, 1.0 % nucleotide substitutions in the small subunit (SSU) rRNA gene and 8.0 % nucleotide substitutions in the internal transcribed spacer (ITS) region. The phylogenetic analyses based on the combined sequences of the SSU and the D1/D2 region and that of the SSU sequences alone confirmed the placement of the novel species in the Nakazawaea clade and its close affinity with N. laoshanensis. Hence, the species Nakazawaea todaengensis f.a., sp. nov. is proposed. The type strain is DMKU-PS11(1)T (=CBS 14555T=TBRC 6559T). The MycoBank number for Nakazawaea todaengensis f.a., sp. nov. is MB 819513.
菌株DMKU - PS11(1)T是从泰国一片沼泽森林的泥炭中分离得到的。DNA序列分析表明，它属于一个新物种，与老挝中泽酵母（Nakazawaea laoshanensis）关系最为密切。然而，它与老挝中泽酵母模式菌株（NRRL Y - 63634T）在大亚基（LSU）rRNA基因的D1/D2区域有2.3%的核苷酸替换，在小亚基（SSU）rRNA基因有1.0%的核苷酸替换，在内转录间隔区（ITS）有8.0%的核苷酸替换。基于SSU和D1/D2区域的联合序列以及单独的SSU序列进行的系统发育分析证实了该新物种在中泽酵母属分支中的位置及其与老挝中泽酵母的密切亲缘关系。因此，提出新物种托登中泽酵母（Nakazawaea todaengensis f.a., sp. nov.）。模式菌株为DMKU - PS11(1)T（=CBS 14555T=TBRC 6559T）。托登中泽酵母（Nakazawaea todaengensis f.a., sp. nov.）的MycoBank编号为MB 819513。
