Farias J G, Zepeda A, Castillo R, Figueroa E, Ademoyero O T, Pulgar V M
Departamento de Ingeniería Química, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Temuco, Chile.
Programa de Fisiopatología, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Andrologia. 2018 Mar;50(2). doi: 10.1111/and.12876. Epub 2017 Jul 31.
Hypobaric hypoxia induces DNA damage in rat testicular cells, the production of defective spermatozoids and decreased sperm count, associated with an increase in oxidative stress. 8-Oxoguanine glycosylase (OGG1) enzymes are main members of the base excision repair (BER) system, a DNA repair mechanism. We determined the expression levels of mitochondrial and nuclear OGG1 isoforms in spermatozoa collected from cauda epididymis in rats exposed to chronic hypobaric hypoxia (CHH) for 5, 15 and 30 days. CHH attenuates OGG1 expression in a time-dependent fashion, with a greater reduction in the mitochondrial isoform OGG1-2a (p < .05). Attenuation of the BER system may contribute to DNA damage under hypoxia exposure.
低压缺氧会导致大鼠睾丸细胞中的DNA损伤、产生有缺陷的精子以及精子数量减少，同时伴随着氧化应激的增加。8-氧代鸟嘌呤糖基化酶（OGG1）是碱基切除修复（BER）系统的主要成员，BER是一种DNA修复机制。我们测定了暴露于慢性低压缺氧（CHH）5天、15天和30天的大鼠附睾尾部采集的精子中线粒体和细胞核OGG1亚型的表达水平。CHH以时间依赖性方式减弱OGG1的表达，线粒体亚型OGG1-2a的减少更为明显（p < 0.05）。BER系统的减弱可能导致缺氧暴露下的DNA损伤。
