高原和低海拔藏猪精子的比较蛋白质组学分析。
Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes.
机构信息
College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, Tibet, 860000, People's Republic of China.
State Key Laboratory of Subtropical Agro-Bioresource Conservation and Utilization, Guangxi University, Nanning, Guangxi Province, 530004, People's Republic of China.
出版信息
BMC Genomics. 2019 Jul 10;20(1):569. doi: 10.1186/s12864-019-5873-0.
BACKGROUND
To illuminate the mechanisms underlying the high-altitude tolerance of Tibetan pig spermatozoa, proteomes of spermatozoa from Tibetan pigs raised in high and low altitudes were compared using a tandem mass tag (TMT)-labeled quantitative proteomics approach.
RESULTS
A total of 77 differentially expressed proteins (DEPs) were identified. Gene Ontology (GO) analysis revealed DEPs that were predominantly associated with the actin cytoskeleton, the tricarboxylic acid (TCA) cycle, and adenosine triphosphate (ATP) metabolism, and were from 12 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Three subnetworks were significantly enriched and 10 centric proteins were identified by protein-protein interaction (PPI) network analysis. Relative expression levels of the proteins (ATP5H, CYCS, MYH9 and FN1) were confirmed using Western blotting.
CONCLUSIONS
Our study is the first to use a tandem mass tag (TMT) approach to analyze Tibetan pig spermatozoa, and provides a foundation to understand the mechanisms underlying the reproductive adaptations of Tibetan pigs to high-altitude environments.
背景
为了阐明藏猪精子高原适应的机制，我们采用串联质量标签（TMT）标记定量蛋白质组学方法比较了来自高海拔和低海拔地区饲养的藏猪精子的蛋白质组。
结果
共鉴定出 77 个差异表达蛋白（DEPs）。基因本体论（GO）分析显示，DEPs 主要与肌动蛋白细胞骨架、三羧酸（TCA）循环和三磷酸腺苷（ATP）代谢有关，并且富集了 12 个京都基因与基因组百科全书（KEGG）途径。通过蛋白质-蛋白质相互作用（PPI）网络分析，发现了三个显著富集的子网络和 10 个中心蛋白。使用 Western blot 验证了这些蛋白质（ATP5H、CYCS、MYH9 和 FN1）的相对表达水平。
结论
本研究首次采用串联质量标签（TMT）方法分析藏猪精子，为理解藏猪生殖适应高原环境的机制提供了基础。
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