Garry D J, Sorenson R L, Elde R P, Maley B E, Madsen A
Diabetes. 1986 Oct;35(10):1090-5. doi: 10.2337/diab.35.10.1090.
gamma-Aminobutyric acid (GABA) is found in high concentrations in the pancreatic islet. In addition, enzymes regulating the level of GABA (L-glutamate decarboxylase and GABA-alpha-ketoglutarate transaminase) have been immunohistochemically localized in the medullary cells of the islet. In this study, an immunofluorescence and elution/restaining protocol is used to determine the distribution of GABA and either insulin, glucagon, or somatostatin in a tissue section. GABA was not detected within the islet alpha- or delta-cells but was determined to be localized within the insulin-containing beta-cells.
γ-氨基丁酸（GABA）在胰岛中含量很高。此外，调节GABA水平的酶（L-谷氨酸脱羧酶和GABA-α-酮戊二酸转氨酶）已通过免疫组织化学定位在胰岛的髓样细胞中。在本研究中，采用免疫荧光和洗脱/复染方案来确定组织切片中GABA与胰岛素、胰高血糖素或生长抑素的分布。在胰岛α细胞或δ细胞内未检测到GABA，但确定其定位于含胰岛素的β细胞内。
