Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
Sci Rep. 2016 Sep 21;6:33884. doi: 10.1038/srep33884.
We previously reported that germline apoptosis in C. elegans increased by loss of PGL-1 and PGL-3, members of a family of constitutive germ-granule components, from germ cells in adult hermaphrodite gonads. In this study, we found that somatic apoptosis was reduced in synthetic multivulva class B (synMuv B) mutants due to ectopic expression of PGL-1 and PGL-3 in the soma. In synMuv B-mutant somatic cells, CED-4 expression level was reduced due to ectopic expression of PGL-1. Furthermore, in contrast to wild type, somatic apoptosis in synMuv B mutants increased following DNA damage in a SIR-2.1-dependent manner. Intriguingly, somatic apoptosis was repressed not only in synMuv B mutants but also by ectopically expressing pgl-1 and/or pgl-3 transgenes in wild-type somatic cells. Our study demonstrates that germ-granule components, PGL-1 and PGL-3, can serve as negative regulators of apoptosis not only in the germline but also in the soma in C. elegans.
我们之前曾报道过，组成生殖质颗粒的组成成分家族成员 PGL-1 和 PGL-3 从成年雌雄同体生殖腺的生殖细胞中缺失后，线虫的生殖细胞凋亡增加。在这项研究中，我们发现由于 PGL-1 和 PGL-3 在体细胞中的异位表达，合成多育突变体 B（synMuv B）中的体细胞凋亡减少。在 synMuv B 突变体细胞中，由于 PGL-1 的异位表达，CED-4 的表达水平降低。此外，与野生型相比，synMuv B 突变体中的体细胞凋亡在 SIR-2.1 依赖性方式下增加，这是由于 DNA 损伤所致。有趣的是，体细胞凋亡不仅在 synMuv B 突变体中受到抑制，而且在野生型体细胞中异位表达 pgl-1 和/或 pgl-3 转基因也受到抑制。我们的研究表明，生殖质颗粒成分 PGL-1 和 PGL-3 不仅可以作为线虫生殖细胞凋亡的负调节剂，而且可以作为体细胞凋亡的负调节剂。
