Shaw T J, Letourneau P C
J Neurosci Res. 1986;16(2):337-55. doi: 10.1002/jnr.490160202.
Adrenal glands from embryonic day 11 (E-11) chicks were cryostat-sectioned, and it was determined that tyrosine hydroxylase-like immunoreactive (TLI) cells, somatostatin-like immunoreactive (SLI) cells, and methionine-enkephalin-like immunoreactive (ELI) cells occupied chromaffin regions of the gland. Similar age adrenals were dissociated, and the cells were cultured under serum-free conditions. Cultured TLI cells, ELI cells, and SLI cells were characterized according to cell size, cell number, and neurite formation. ELI and SLI cells composed two largely separate populations, with SLI cells tending to have larger cell areas, to be more numerous, and to be less likely to form neurites than ELI cells. The population of TLI cells, although unique in itself, was diverse and numerous enough to include all or portions of the neuropeptide-immunoreactive populations. Neurites of some cells from each of the above populations were strongly immunoreactive for alpha neurofilament protein, and for NAPA73 neurofilament-associated protein. However, neurites could also be observed in all populations that showed poor immunoreactivity for these cytoskeletal proteins. Exogenously added NGF significantly increased neurite-like process formation among TLI and ELI cells, but not among SLI cells. Reductions in the number of neurite-like processes following treatment with anti-nerve growth factor (NGF) were not significant for any of the populations. However, if shorter and broader process were included, anti-NGF caused a significant reduction in total cell processes among TLI and ELI cells. Anti-NGF inhibition of process formation among ELI cells could be reversed with exogenous NGF. Neither NGF or anti-NGF treatments showed a significant effect on cell numbers among TLI and ELI populations. The implications are that a compound of antigenic and physiological similarity to mouse salivary NGF is made by embryonic chick adrenal cells in culture, but the effects of NGF do not appear to be the same for all neural-crest-derived cells from the adrenal, and greater heterogeneity of phenotypes may exist among chromaffin cells than has previously been accepted. Some questions are also raised concerning the neurite-like nature of processes formed by some chromaffin cells in vitro.
对胚胎第11天（E-11）雏鸡的肾上腺进行冰冻切片，结果显示酪氨酸羟化酶样免疫反应性（TLI）细胞、生长抑素样免疫反应性（SLI）细胞和甲硫氨酸脑啡肽样免疫反应性（ELI）细胞占据了肾上腺的嗜铬区域。将相似年龄的肾上腺进行解离，并在无血清条件下培养细胞。根据细胞大小、细胞数量和神经突形成对培养的TLI细胞、ELI细胞和SLI细胞进行鉴定。ELI细胞和SLI细胞构成两个基本分开的群体，与ELI细胞相比，SLI细胞往往具有更大的细胞面积、更多的细胞数量，且形成神经突的可能性更小。TLI细胞群体虽然本身独特，但种类繁多，足以包含全部或部分神经肽免疫反应性群体。上述每个群体中一些细胞的神经突对α神经丝蛋白和NAPA73神经丝相关蛋白具有强烈的免疫反应性。然而，在所有对这些细胞骨架蛋白免疫反应性较差的群体中也能观察到神经突。外源性添加神经生长因子（NGF）显著增加了TLI细胞和ELI细胞中神经突样突起的形成，但对SLI细胞没有影响。用抗神经生长因子（NGF）处理后，神经突样突起数量的减少对任何群体都不显著。然而，如果将更短更宽的突起包括在内，抗NGF会导致TLI细胞和ELI细胞中总细胞突起显著减少。外源性NGF可逆转抗NGF对ELI细胞突起形成的抑制作用。NGF或抗NGF处理对TLI细胞和ELI细胞群体中的细胞数量均无显著影响。这意味着培养的胚胎雏鸡肾上腺细胞会产生一种与小鼠唾液NGF具有抗原和生理相似性的化合物，但NGF对肾上腺所有神经嵴衍生细胞的作用似乎并不相同，嗜铬细胞中的表型异质性可能比以前认为的更大。同时也引发了一些关于体外某些嗜铬细胞形成的突起样结构性质的问题。
