Tischler A S, DeLellis R A, Slayton V W, Blount M W, Wolfe H J
Lab Invest. 1983 Jan;48(1):13-8.
Normal adult human chromaffin cells in culture contain abundant immunoreactive enkephalin and exhibit spontaneous and nerve growth factor-induced outgrowth of processes. In cultures of adrenals from two age-matched white males, approximately 70 per cent of chromaffin cells from one case and 50 per cent from the other contained immunocytochemically demonstrable leuenkephalin-like material. There was no apparent correlation between enkephalin-like immunoreactivity and capacity for process outgrowth. Cells with and without processes stained for enkephalin, and the percentages of stained cells with processes appeared to change in proportion of overall process outgrowth. Numerous cells with and without processes also failed to stain for enkephalin. Marked variations in sizes of stained cells and in distribution and intensity of staining were observed in all of the cultures. These findings suggest that adult chromaffin cells are heterogeneous at a number of levels.
培养的正常成人嗜铬细胞含有丰富的免疫反应性脑啡肽，并表现出突起的自发生长和神经生长因子诱导的生长。在两名年龄匹配的白人男性的肾上腺培养物中，一例中约70%的嗜铬细胞和另一例中50%的嗜铬细胞含有免疫细胞化学可证实的亮脑啡肽样物质。脑啡肽样免疫反应性与突起生长能力之间没有明显的相关性。有突起和无突起的细胞都能被脑啡肽染色，有突起的染色细胞百分比似乎随着整体突起生长比例的变化而变化。许多有突起和无突起的细胞也未能被脑啡肽染色。在所有培养物中均观察到染色细胞大小、染色分布和强度的显著差异。这些发现表明，成人嗜铬细胞在多个水平上是异质性的。
