Unsicker K, Rieffert B, Ziegler W
Adv Biochem Psychopharmacol. 1980;25:51-9.
Adrenal chromaffin cells from young rats, newborn guinea pigs, and adult cattle were grown under various cell culture conditions. Nerve growth factor (NGF) elicited the outgrowth of neurite-like processes from rat, but not from bovine and guinea pig, chromaffin cells. Dexamethasone, dibutyryl cAMP, theophylline, and cholera toxin, which are known to augment intracellular cAMP, specifically inhibited neurite outgrowth. We propose that glucocorticoid hormones and mechanisms which act through an increase of cAMP are prerequisites for the acquisition and maintenance of differentiation and expression of an endocrine instead of a neuronal phenotype of adrenal chromaffin cells.
将来自幼鼠、新生豚鼠和成年牛的肾上腺嗜铬细胞置于各种细胞培养条件下培养。神经生长因子（NGF）能诱导大鼠嗜铬细胞长出类神经突，但不能诱导牛和豚鼠嗜铬细胞长出类神经突。已知可增强细胞内cAMP的地塞米松、二丁酰cAMP、茶碱和霍乱毒素可特异性抑制神经突生长。我们认为，糖皮质激素以及通过增加cAMP起作用的机制是肾上腺嗜铬细胞获得并维持内分泌而非神经元表型的分化和表达的先决条件。
