Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.
Intensive Care Medicine, Nijmegen Institute for Infection, Inflammation and Immunity, Radboud University Medical Center, Nijmegen, The Netherlands.
Sci Rep. 2017 Jul 31;7(1):6889. doi: 10.1038/s41598-017-05930-9.
Ethylene is a major plant hormone mediating developmental processes and stress responses to stimuli such as infection. We show here that ethylene is also produced during systemic inflammation in humans and is released in exhaled breath. Traces of ethylene were detected by laser spectroscopy both in vitro in isolated blood leukocytes exposed to bacterial lipopolysaccharide (LPS) as well as in vivo following LPS administration in healthy volunteers. Exposure to LPS triggers formation of ethylene as a product of lipid peroxidation induced by the respiratory burst. In humans, ethylene was detected prior to the increase of blood levels of inflammatory cytokines and stress-related hormones. Our results highlight that ethylene release is an early and integral component of in vivo lipid peroxidation with important clinical implications as a breath biomarker of bacterial infection.
乙烯是一种主要的植物激素，介导发育过程和对刺激（如感染）的应激反应。我们在这里表明，乙烯也在人类系统性炎症中产生，并在呼出的呼吸中释放。通过激光光谱法，我们在体外分离的血液白细胞暴露于细菌脂多糖（LPS）以及健康志愿者给予 LPS 后体内均检测到了乙烯的痕迹。LPS 的暴露会触发作为呼吸爆发诱导的脂质过氧化产物的乙烯的形成。在人类中，乙烯在血液中炎症细胞因子和应激相关激素水平升高之前就被检测到了。我们的结果强调了乙烯的释放是体内脂质过氧化的早期和整体组成部分，作为细菌感染的呼吸生物标志物具有重要的临床意义。
