Carmona Jorge U, Ríos Diana L, López Catalina, Álvarez María E, Pérez Jorge E
Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
Grupo de Investigación Biosalud, Departamento de Ciencias Básicas para la Salud, Universidad de Caldas, Manizales, Colombia.
Vet Med Int. 2017;2017:6059485. doi: 10.1155/2017/6059485. Epub 2017 Jul 6.
Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane. The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS). SMEs from six horses were cultured over 96 h. Then, SMEs were harvested for RNA extraction and quantitative gene expression analysis by RT-qPCR for nuclear factor kappa B (NFB), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1), and cartilage oligomeric matrix protein (COMP). The 25% and 50% Lc-PRG supernatants led to downregulation of NFB, MMP-13, ADAMTS-4, COL1A1, COL2A1, and COMP in SMEs. Lr-PRG supernatants (particularly at the 50% concentration) induced downregulation of NFB, MMP-13, ADAMTS-4, and COL1A1 and upregulation of COL2A1 and COMP. Lr-PRG supernatants should be used for the treatment of inflammatory arthropathies in horses because they have anti-inflammatory and anabolic effects in the synovial membrane.
富含血小板血浆（PRP）制剂用于患骨关节炎（OA）的马匹。然而，关于产生足够的抗炎和合成代谢反应以作用于滑膜的血小板和白细胞的理想浓度仍存在一些争议。本研究的目的是研究富含白细胞的血小板凝胶（Lc-PRG）和去白细胞的血小板凝胶（Lr-PRG）上清液对用脂多糖（LPS）刺激的马滑膜外植体（SMEs）中一些促炎和合成代谢基因定量表达的影响。来自六匹马的SMEs培养96小时。然后，收集SMEs用于RNA提取，并通过RT-qPCR对核因子κB（NFB）、基质金属蛋白酶13（MMP-13）、含血小板反应蛋白基序的解聚素和金属蛋白酶4（ADAMTS-4）、I型胶原蛋白α1（COL1A1）、II型胶原蛋白α1（COL2A1）和软骨寡聚基质蛋白（COMP）进行定量基因表达分析。25%和50%的Lc-PRG上清液导致SMEs中NFB、MMP-13、ADAMTS-4、COL1A1、COL2A1和COMP的下调。Lr-PRG上清液（特别是50%浓度时）诱导NFB、MMP-13、ADAMTS-4和COL1A1的下调以及COL2A1和COMP的上调。Lr-PRG上清液应用于治疗马的炎性关节病，因为它们在滑膜中具有抗炎和合成代谢作用。
