Doostan Farideh, Vafafar Roxana, Zakeri-Milani Parvin, Pouri Aliasghar, Amini Afshar Rogayeh, Mesgari Abbasi Mehran
Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Biology, Faculty of Science, Islamic Azad University, Ahar Branch, Ahar, Iran.
Adv Pharm Bull. 2017 Jun;7(2):269-274. doi: 10.15171/apb.2017.032. Epub 2017 Jun 30.
Methotrexate (MTX) is prescribed in many diseases and can result in oxidative stress (OS) followed by injuries in some tissues. Antioxidants administration are effective in reducing OS. Pomegranate exhibits high anti-oxidant capacities. This study investigated whether pomegranate seed and peel methanolic extracts (PSE and PPE) could protect against MTX-induced OS and lipid profile changes in rats. Forty-eight rats were randomly divided into 6 groups: control group (normal salin), PSE group (500 mg/kg, orally), PPE group (500 mg/kg, orally), MTX group (10 mg/kg, IM), MTX and PSE group, and MTX and PPE group. Blood samples were taken for analysis in the end of the procedure. The findings showed a significant reduction in Glutathione peroxidase (GPx) and Superoxide dismutase (SOD), and an enhancement in malondialdehyde (MDA) values after MTX treatment (p < 0.05). SOD and GPx levels reached the levels of the control group in MTX+SPE and MTX+PPE groups. No significant differences were observed in catalase (CAT) and total antioxidant capacity (TAC) levels between groups. The results showed a significant decrease in total cholesterol (TC), low density lipoprotein (LDL), and high density lipoprotein (HDL) in the MTX treated group (p < 0.01). The values of TC, HDL, and LDL became elevated to the normal control levels in the MTX+PSE and MTX+PPE treated groups. The results showed the OS induced by MTX and the protective effects of PSE and PPE against MTX-induced serum oxidative stress and lipid profile changes in rats.
甲氨蝶呤（MTX）被用于多种疾病的治疗，可能会导致氧化应激（OS），进而引起某些组织的损伤。给予抗氧化剂可有效减轻氧化应激。石榴具有很高的抗氧化能力。本研究调查了石榴籽和石榴皮甲醇提取物（PSE和PPE）是否能预防MTX诱导的大鼠氧化应激和脂质谱变化。48只大鼠随机分为6组：对照组（生理盐水）、PSE组（500毫克/千克，口服）、PPE组（500毫克/千克，口服）、MTX组（10毫克/千克，肌肉注射）、MTX与PSE组以及MTX与PPE组。实验结束时采集血样进行分析。结果显示，MTX治疗后谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）显著降低，丙二醛（MDA）值升高（p<0.05）。MTX+SPE组和MTX+PPE组的SOD和GPx水平达到了对照组水平。各组之间过氧化氢酶（CAT）和总抗氧化能力（TAC）水平未观察到显著差异。结果显示，MTX治疗组的总胆固醇（TC）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）显著降低（p<0.01）。MTX+PSE组和MTX+PPE治疗组中TC、HDL和LDL的值升高至正常对照水平。结果表明MTX可诱导氧化应激，PSE和PPE对MTX诱导的大鼠血清氧化应激和脂质谱变化具有保护作用。
