Department of Biochemistry, University of Allahabad, Allahabad, Uttar Pradesh, India.
Pharm Biol. 2013 Apr;51(4):441-6. doi: 10.3109/13880209.2012.738333. Epub 2013 Jan 22.
Pomegranate [Punica granatum L. (Lythraceae)] is rich in antioxidants.
The present work was undertaken to evaluate the protective effects of methanol extract of pomegranate peel extract (PPE) against a rat model of oxidative stress generated by treatment with mercuric chloride (HgCl(2)).
Rats were injected with HgCl(2) (5 mg/kg body weight in 0.9% NaCl) for developing oxidative stress. The methanol extract of pomegranate peel (PPE) was suspended in 0.5% sodium carboxymethylcellulose and fed to rats at 50 mg/kg body weight/day up to 30 days after HgCl(2) treatment. Biomarkers of oxidative stress including erythrocyte plasma membrane redox system (PMRS), lipid peroxidation, advanced oxidation protein products (AOPP), intracellular glutathione, and plasma antioxidant capacity as FRAP values were measured after PPE supplementation.
HgCl(2)-treated rats showed increased oxidative stress as evidenced by increased activity of erythrocyte PMRS 153%, lipid peroxidation 31%, and AOPP 290%. There was a significant (p < 0.01) decrease (25%) in plasma antioxidant capacity and intracellular glutathione (75%). Supplementation with PPE protected rats from HgCl(2) induced oxidative damage. PPE supplementation to control rats increased antioxidant defense.
The results clearly demonstrate that PPE treatment augments the antioxidant defense mechanism in mercuric chloride-induced oxidant toxicity. To the best of our knowledge this is the first report of the increase in erythrocyte PMRS activity in rats after supplementation with any kind of antioxidant supplementation. This study emphasizes the need for characterizing PPE for potential unknown antioxidant compounds.
石榴（Punica granatum L.（石榴科））富含抗氧化剂。
本研究旨在评估石榴皮甲醇提取物（PPE）对氯化汞（HgCl 2 ）处理大鼠氧化应激模型的保护作用。
大鼠注射 HgCl 2 （5mg/kg 体重，0.9%NaCl）以产生氧化应激。将石榴皮甲醇提取物（PPE）悬浮在 0.5%羧甲基纤维素钠中，在 HgCl 2 处理后 30 天内以 50mg/kg 体重/天的剂量给予大鼠。补充 PPE 后，测定氧化应激生物标志物，包括红细胞质膜氧化还原系统（PMRS）、脂质过氧化、高级氧化蛋白产物（AOPP）、细胞内谷胱甘肽和血浆抗氧化能力（FRAP 值）。
HgCl 2 处理大鼠的红细胞 PMRS 活性增加 153%、脂质过氧化增加 31%、AOPP 增加 290%，表明氧化应激增加。血浆抗氧化能力和细胞内谷胱甘肽分别显著降低（p < 0.01）25%和 75%。PPE 补充可保护大鼠免受 HgCl 2 诱导的氧化损伤。PPE 补充对对照组大鼠的抗氧化防御能力有增强作用。
结果清楚地表明，PPE 治疗增强了氯化汞诱导的氧化毒性中的抗氧化防御机制。据我们所知，这是首次报道大鼠补充任何类型的抗氧化剂后红细胞 PMRS 活性增加。本研究强调需要对 PPE 进行特征分析，以发现潜在的未知抗氧化化合物。
