Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China.
Department of Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China.
Oncol Res. 2018 Mar 5;26(2):289-296. doi: 10.3727/096504017X15009404458675. Epub 2017 Jul 25.
Long noncoding RNAs (lncRNAs) have been implicated in various biological processes and pathological conditions, including tumorigenesis. However, the exact roles of NEAT1 and its underlying mechanisms in non-small cell lung cancer (NSCLC) remain largely unclear. In the present study, lncRNA NEAT1 was detected to be significantly upregulated in NSCLC tissues and closely associated with advanced TNM stages, lymph node metastasis, distant metastasis, and poor prognosis. Further experiments revealed that lncRNA NEAT1 silencing inhibited cell proliferation and invasion in vitro. In addition, mechanistic analysis showed that lncRNA NEAT1 upregulated the miR-181a-5p-targeted gene HMGB2 through acting as a competitive "sponge" of miR-181a-5p. In conclusion, our study suggested that lncRNA NEAT1 plays an oncogenic role in NSCLC progression and provides potential mechanisms by which lncRNA NEAT1 contributes to this disease.
长链非编码 RNA（lncRNA）在多种生物学过程和病理条件中发挥作用，包括肿瘤发生。然而，NEAT1 的确切作用及其在非小细胞肺癌（NSCLC）中的潜在机制仍很大程度上不清楚。在本研究中，检测到 lncRNA NEAT1 在 NSCLC 组织中显著上调，与晚期 TNM 分期、淋巴结转移、远处转移和不良预后密切相关。进一步的实验表明，lncRNA NEAT1 沉默抑制了体外细胞增殖和侵袭。此外，机制分析表明，lncRNA NEAT1 通过作为 miR-181a-5p 的竞争性“海绵”来上调 miR-181a-5p 靶向基因 HMGB2。总之，本研究表明 lncRNA NEAT1 在 NSCLC 进展中发挥致癌作用，并提供了 lncRNA NEAT1 促进该疾病的潜在机制。
