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肺鳞状细胞癌诊断和预后生物标志物的鉴定与验证

Identification and validation of diagnostic and prognostic biomarkers for lung squamous cell carcinoma.

作者信息

Zhao Xiaopeng, Yuan Chong, He Xu, Wang Miao, Zhang Haoran, Cheng Jingge, Wang Hongyan

机构信息

Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.

The First Outpatient Department Directly Under Hebei Province, Shijiazhuang, China.

出版信息

J Thorac Dis. 2022 Apr;14(4):1243-1255. doi: 10.21037/jtd-22-343.

DOI:10.21037/jtd-22-343
PMID:35572889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9096289/
Abstract

BACKGROUND

The aim of the present study was to find diagnostic and prognostic biomarkers for lung squamous cell carcinoma (LUSC) and to validate key biomarkers .

METHODS

RNA sequencing was used to identify differentially expressed mRNAs (DEmRNAs) and differentially expressed long non-coding RNAs (DElncRNAs) in LUSC tissues. RNA sequencing results were validated using a published dataset. Diagnostic and prognostic values of candidate genes were evaluated by receiver-operating characteristic (ROC) curve analysis and survival analysis, respectively. To determine the effect of in LUSC, expression was knocked down in NCI-H520 cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Transwell assay were used to respectively detect the effect of on cell proliferation and migration.

RESULTS

In total, 1,946 DEmRNAs and 428 DElncRNAs were identified in LUSC compared with normal tissues. A total of 851 DElncRNA-DEmRNA co-expression pairs were obtained. With the exception of and were upregulated in LUSC. ROC curve analysis indicated that , and could predict LUSC. Survival analysis suggested that , and had potential prognostic value for LUSC. knockdown inhibited cell proliferation and migration, and significantly reduced the expression of .

CONCLUSIONS

The findings of the present study could help determine the pathogenesis of LUSC and provide new and accurate therapeutic targets for its treatment.

摘要

背景

本研究旨在寻找肺鳞状细胞癌（LUSC）的诊断和预后生物标志物，并验证关键生物标志物。

方法

采用RNA测序技术鉴定LUSC组织中差异表达的mRNA（DEmRNAs）和差异表达的长链非编码RNA（DElncRNAs）。利用已发表的数据集对RNA测序结果进行验证。分别通过受试者工作特征（ROC）曲线分析和生存分析评估候选基因的诊断和预后价值。为了确定[未提及基因名称]在LUSC中的作用，在NCI-H520细胞中敲低其表达。采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法和Transwell法分别检测[未提及基因名称]对细胞增殖和迁移的影响。

结果

与正常组织相比，LUSC中共鉴定出1946个DEmRNAs和428个DElncRNAs。共获得851个DElncRNA-DEmRNA共表达对。除[未提及基因名称]外，[未提及基因名称]在LUSC中上调。ROC曲线分析表明，[未提及基因名称]可预测LUSC。生存分析提示，[未提及基因名称]对LUSC具有潜在的预后价值。[未提及基因名称]敲低抑制细胞增殖和迁移，并显著降低[未提及基因名称]的表达。

结论

本研究结果有助于确定LUSC的发病机制，并为其治疗提供新的、准确的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e81e/9096289/6a63a5659f11/jtd-14-04-1243-f10.jpg
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