Wang-Leandro A, Hobert M K, Alisauskaite N, Dziallas P, Rohn K, Stein V M, Tipold A
Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Hannover, Germany.
Centre for Systems Neuroscience, Hannover, Germany.
Spinal Cord. 2017 Dec;55(12):1108-1116. doi: 10.1038/sc.2017.83. Epub 2017 Aug 1.
Prospective observational-analytical study.
Description of diffusion tensor imaging (DTI) metrics obtained from the spinal cord (SC) of dogs with severe acute or chronic spontaneous, non-experimentally induced spinal cord injury (SCI) and correlation of DTI values with lesion extent of SCI measured in T2-weighted (T2W) magnetic resonance imaging sequences.
Hannover, Germany.
Forty-seven paraplegic dogs, 32 with acute and 15 with chronic SCI, and 6 disease controls were included. T2W and DTI sequences of the thoracolumbar spinal cord were performed. Values of fractional anisotropy (FA) and apparent diffusion coefficient (ADC) were obtained from the epicentre of the lesion and one SC segment cranially and caudally and compared between groups. Pearson's correlation coefficient was calculated between DTI and T2W metrics.
During acute SCI, FA values were increased (P=0.0065) and ADC values were decreased (P=0.0099) at epicentres compared to disease controls. FA values obtained from dogs with chronic SCI were lower (P<0.0001 epicentres and caudally; P=0.0002 cranially) and ADC showed no differences compared to disease control values. Dogs with chronic SCI revealed lower FA and higher ADC compared to dogs with acute SCI (P<0.0001 for both values at all localisations). FA values from epicentre and cranially to the lesion during chronic SCI correlated with extent of lesion (r=0.5517; P=0.0052 epicentres and r=0.6810; P=0.0408 cranially).
Using DTI, differences between acute and chronic stages of spontaneous canine SCI were detected and correlations between T2W and DTI sequences were found in chronic SCI, supporting canine SCI as a useful large animal model.
前瞻性观察分析研究。
描述从患有严重急性或慢性自发性、非实验性诱导脊髓损伤（SCI）的犬类脊髓（SC）获得的扩散张量成像（DTI）指标，并将DTI值与在T2加权（T2W）磁共振成像序列中测量的SCI病变范围进行相关性分析。
德国汉诺威。
纳入47只截瘫犬，其中32只患有急性SCI，15只患有慢性SCI，以及6只疾病对照犬。对胸腰段脊髓进行T2W和DTI序列检查。从病变中心以及头侧和尾侧的一个脊髓节段获取分数各向异性（FA）值和表观扩散系数（ADC）值，并在组间进行比较。计算DTI和T2W指标之间的Pearson相关系数。
在急性SCI期间，与疾病对照相比，病变中心的FA值升高（P = 0.0065），ADC值降低（P = 0.0099）。慢性SCI犬获得的FA值较低（病变中心和尾侧P < 0.0001；头侧P = 0.0002），与疾病对照值相比，ADC无差异。与急性SCI犬相比，慢性SCI犬的FA较低，ADC较高（所有部位的两个值均P < 0.0001）。慢性SCI期间病变中心和病变头侧的FA值与病变范围相关（r = 0.5517；病变中心P = 0.0052，r = 0.6810；头侧P = 0.0408）。
使用DTI，检测到自发性犬SCI急性和慢性阶段之间的差异，并且在慢性SCI中发现了T2W和DTI序列之间的相关性，支持犬SCI作为一种有用的大型动物模型。
