Brodie Tess Melinda, Tosevski Vinko
University of Zurich, Mass Cytometry Facility, Zurich, Switzerland.
Curr Protoc Immunol. 2017 Aug 1;118:5.11.1-5.11.25. doi: 10.1002/cpim.31.
Mass cytometry is an analytical technology that combines the sample preparation workflow typical of flow cytometry and the detection capacity of atomic mass spectroscopy, allowing for highly multiplexed measurements of protein or nucleic acid targets on single cells. In 2014, the mass cytometer was adapted for the acquisition of samples from microscopy slides (termed imaging mass cytometry), greatly increasing the applicability of this technology. By using antibodies (or other probes) labeled with purified metal isotopes, the mass cytometer is able to detect up to 50 different parameters (current practical limit) at the single-cell level, enabling a deep and thorough profiling of individual cells in terms of their cell surface protein phenotype, physiological state, proliferation potential, and many other cell states or features. © 2017 by John Wiley & Sons, Inc.
质谱流式细胞术是一种分析技术，它将流式细胞术典型的样品制备流程与原子质量光谱的检测能力相结合，能够对单细胞上的蛋白质或核酸靶标进行高度多重测量。2014年，质谱流式细胞仪被用于从显微镜载玻片采集样品（称为成像质谱流式细胞术），大大提高了该技术的适用性。通过使用标记有纯化金属同位素的抗体（或其他探针），质谱流式细胞仪能够在单细胞水平检测多达50个不同参数（当前实际限制），从而能够根据单个细胞的细胞表面蛋白表型、生理状态、增殖潜力以及许多其他细胞状态或特征，对其进行深入而全面的分析。© 2017约翰威立父子公司版权所有
