Heck Susanne, Bishop Cynthia Jane, Ellis Richard Jonathan
NIHR Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.
Methods Mol Biol. 2019;1979:285-303. doi: 10.1007/978-1-4939-9240-9_18.
Mass cytometry is a variation of conventional flow cytometry using metal tagged antibodies for cell staining instead of fluorochromes and detection in a mass cytometer, a modified mass spectrometer that allows for separation of discrete masses of these metal tags by time of flight (TOF). Currently, up to 50 different metal tags are available for cell analysis. The lack of any significant mass spectral overlap and autofluorescence background makes mass cytometry uniquely suited for complex high-dimensional phenotypic and functional analysis at the single cell level, thus accelerating biomarker discovery and drug screening. Here we describe a workflow for phenotyping of human peripheral blood mononuclear cells (PBMCs) covering cell staining, instrument setup of a Fluidigm Helios™ mass cytometer, and sample acquisition, and summarize a basic workflow of data analysis.
质谱流式细胞术是传统流式细胞术的一种变体，它使用金属标记抗体而非荧光染料对细胞进行染色，并在质谱细胞仪中进行检测。质谱细胞仪是一种经过改良的质谱仪，可通过飞行时间（TOF）分离这些金属标记的离散质量。目前，有多达50种不同的金属标记可用于细胞分析。由于不存在明显的质谱重叠和自发荧光背景，质谱流式细胞术特别适用于单细胞水平的复杂高维表型和功能分析，从而加速生物标志物发现和药物筛选。在此，我们描述了一种用于人外周血单核细胞（PBMC）表型分析的工作流程，涵盖细胞染色、Fluidigm Helios™ 质谱细胞仪的仪器设置和样本采集，并总结了数据分析的基本工作流程。
