• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

重组痘苗病毒的产生

Generation of Recombinant Vaccinia Viruses.

作者信息

Wyatt Linda S, Earl Patricia L, Moss Bernard

机构信息

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.

出版信息

Curr Protoc Protein Sci. 2017 Aug 1;89:5.13.1-5.13.18. doi: 10.1002/cpps.33.

DOI:10.1002/cpps.33
PMID:28762491
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5765993/
Abstract

This unit describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector or PCR fragment to generate a recombinant virus. Selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses are described. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented. © 2017 by John Wiley & Sons, Inc.

摘要

本单元描述了如何用痘苗病毒感染细胞，然后用质粒转移载体或PCR片段转染细胞以产生重组病毒。还描述了用于分离重组病毒的选择和筛选方法以及重组病毒的扩增方法。最后，介绍了一种主要用于检测重组安卡拉痘苗病毒（MVA）的活细胞免疫染色方法。©2017约翰威立父子公司。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a644/5765993/2d47175ed344/nihms878787f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a644/5765993/36895122a5bf/nihms878787f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a644/5765993/2d47175ed344/nihms878787f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a644/5765993/36895122a5bf/nihms878787f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a644/5765993/2d47175ed344/nihms878787f2.jpg

相似文献

1
Generation of Recombinant Vaccinia Viruses.重组痘苗病毒的产生
Curr Protoc Protein Sci. 2017 Aug 1;89:5.13.1-5.13.18. doi: 10.1002/cpps.33.
2
Generation of Recombinant Vaccinia Viruses.重组痘苗病毒的产生
Curr Protoc Mol Biol. 2017 Jan 5;117:16.17.1-16.17.18. doi: 10.1002/cpmb.32.
3
Generation of recombinant vaccinia viruses.重组痘苗病毒的产生。
Curr Protoc Mol Biol. 2001 May;Chapter 16:Unit16.17. doi: 10.1002/0471142727.mb1617s43.
4
Generation of Recombinant Vaccinia Viruses.重组痘苗病毒的产生
Curr Protoc Microbiol. 2015 Nov 3;39:14A.4.1-14A.4.18. doi: 10.1002/9780471729259.mc14a04s39.
5
Generation of recombinant vaccinia viruses.重组痘苗病毒的产生。
Curr Protoc Protein Sci. 2001 May;Chapter 5:Unit5.13. doi: 10.1002/0471140864.ps0513s13.
6
Easy and efficient protocols for working with recombinant vaccinia virus MVA.用于处理重组痘苗病毒MVA的简便高效方案。
Methods Mol Biol. 2012;890:59-92. doi: 10.1007/978-1-61779-876-4_4.
7
Nonreplicating vaccinia vector efficiently expresses recombinant genes.非复制型痘苗病毒载体能高效表达重组基因。
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10847-51. doi: 10.1073/pnas.89.22.10847.
8
Preparation of Cell Cultures and Vaccinia Virus Stocks.细胞培养物和痘苗病毒储备液的制备。
Curr Protoc Protein Sci. 2017 Aug 1;89:5.12.1-5.12.18. doi: 10.1002/cpps.34.
9
Characterization of recombinant vaccinia viruses and their products.重组痘苗病毒及其产物的特性分析。
Curr Protoc Protein Sci. 2001 May;Chapter 5:Unit5.14. doi: 10.1002/0471140864.ps0514s13.
10
[A novel double expression shuttle vector to get marker-free recombinant modified vaccinia virus Ankara].一种用于获得无标记重组改良痘苗病毒安卡拉的新型双表达穿梭载体
Sheng Wu Gong Cheng Xue Bao. 2011 Jun;27(6):926-34.

引用本文的文献

1
Vaccinia Virus-A Swiss Army Knife Against Cancer.痘苗病毒——对抗癌症的瑞士军刀。
Cancers (Basel). 2025 Jul 12;17(14):2324. doi: 10.3390/cancers17142324.
2
A Multi-Valent Hantavirus Vaccine Based on Recombinant Modified Vaccinia Ankara Reduces Viral Load in a Mouse Infection Model.一种基于重组改良安卡拉痘苗病毒的多价汉坦病毒疫苗可降低小鼠感染模型中的病毒载量。
Vaccines (Basel). 2025 Mar 4;13(3):270. doi: 10.3390/vaccines13030270.
3
Rapid Development of Modified Vaccinia Virus Ankara (MVA)-Based Vaccine Candidates Against Marburg Virus Suitable for Clinical Use in Humans.

本文引用的文献

1
Preparation of Cell Cultures and Vaccinia Virus Stocks.细胞培养物和痘苗病毒储备液的制备。
Curr Protoc Protein Sci. 2017 Aug 1;89:5.12.1-5.12.18. doi: 10.1002/cpps.34.
2
Elucidating and minimizing the loss by recombinant vaccinia virus of human immunodeficiency virus gene expression resulting from spontaneous mutations and positive selection.阐明并最小化重组痘苗病毒因自发突变和正选择导致的人类免疫缺陷病毒基因表达损失。
J Virol. 2009 Jul;83(14):7176-84. doi: 10.1128/JVI.00687-09. Epub 2009 May 6.
3
Selection of transfected mammalian cells.
基于安卡拉痘苗病毒（MVA）的抗马尔堡病毒候选疫苗的快速研发，适用于人类临床应用。
Vaccines (Basel). 2024 Nov 24;12(12):1316. doi: 10.3390/vaccines12121316.
4
Characterization of an African Swine Fever Virus Field Isolate from Vietnam with Deletions in the Left Variable Multigene Family Region.一株来自越南的非洲猪瘟病毒田间分离株的特性研究，该分离株的左可变多基因家族区域存在缺失
Viruses. 2024 Apr 7;16(4):571. doi: 10.3390/v16040571.
5
Efficacy of Fowlpox Virus Vector Vaccine Expressing VP2 and Chicken Interleukin-18 in the Protection against Infectious Bursal Disease Virus.表达VP2和鸡白细胞介素-18的禽痘病毒载体疫苗在预防传染性法氏囊病病毒中的效力
Vaccines (Basel). 2023 Nov 14;11(11):1716. doi: 10.3390/vaccines11111716.
6
Rendezvous with Vaccinia Virus in the Post-smallpox Era: R&D Advances.在后天花时代与牛痘病毒的相会：研发进展。
Viruses. 2023 Aug 15;15(8):1742. doi: 10.3390/v15081742.
7
The Emergence of Monkeypox: A Global Health Threat.猴痘的出现：一种全球健康威胁。
Cureus. 2022 Sep 18;14(9):e29304. doi: 10.7759/cureus.29304. eCollection 2022 Sep.
8
Efficient Method for Generating Point Mutations in the Vaccinia Virus Genome Using CRISPR/Cas9.利用 CRISPR/Cas9 在牛痘病毒基因组中生成点突变的有效方法。
Viruses. 2022 Jul 18;14(7):1559. doi: 10.3390/v14071559.
9
Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies.粘膜疫苗接种在未检测到中和抗体的情况下可诱导针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的保护作用。
NPJ Vaccines. 2021 Nov 29;6(1):139. doi: 10.1038/s41541-021-00405-5.
10
Efficient Selection of Recombinant Fluorescent Vaccinia Virus Strains and Rapid Virus Titer Determination by Using a Multi-Well Plate Imaging System.利用多孔板成像系统高效筛选重组荧光痘苗病毒株并快速测定病毒滴度
Biomedicines. 2021 Aug 18;9(8):1032. doi: 10.3390/biomedicines9081032.
转染哺乳动物细胞的选择。
Curr Protoc Mol Biol. 2009 Apr;Chapter 9:Unit9.5. doi: 10.1002/0471142727.mb0905s86.
4
Calcium phosphate transfection.磷酸钙转染
Curr Protoc Mol Biol. 2003 Aug;Chapter 9:Unit 9.1. doi: 10.1002/0471142727.mb0901s63.
5
Gene expression using the vaccinia virus/T7 RNA polymerase hybrid system.使用痘苗病毒/T7 RNA聚合酶杂交系统进行基因表达。
Curr Protoc Mol Biol. 2001 May;Chapter 16:Unit16.19. doi: 10.1002/0471142727.mb1619s43.
6
Correlation of immunogenicities and in vitro expression levels of recombinant modified vaccinia virus Ankara HIV vaccines.重组安卡拉痘苗病毒HIV疫苗的免疫原性与体外表达水平的相关性
Vaccine. 2008 Jan 24;26(4):486-93. doi: 10.1016/j.vaccine.2007.11.036. Epub 2007 Dec 3.
7
Engineering of a vaccinia virus bacterial artificial chromosome in Escherichia coli by bacteriophage lambda-based recombination.利用基于噬菌体λ的重组技术在大肠杆菌中构建痘苗病毒细菌人工染色体
Nat Methods. 2005 Feb;2(2):95-7. doi: 10.1038/nmeth734.
8
Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice.减毒痘苗病毒表达的严重急性呼吸综合征冠状病毒刺突蛋白对小鼠具有保护性免疫作用。
Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6641-6. doi: 10.1073/pnas.0401939101. Epub 2004 Apr 19.
9
Comparative efficacy of recombinant modified vaccinia virus Ankara expressing simian immunodeficiency virus (SIV) Gag-Pol and/or Env in macaques challenged with pathogenic SIV.表达猿猴免疫缺陷病毒（SIV）Gag-Pol和/或Env的重组改良安卡拉痘苗病毒在感染致病性SIV的猕猴中的比较疗效。
J Virol. 2000 Mar;74(6):2740-51. doi: 10.1128/jvi.74.6.2740-2751.2000.
10
Compact, synthetic, vaccinia virus early/late promoter for protein expression.用于蛋白质表达的紧凑型合成痘苗病毒早期/晚期启动子。
Biotechniques. 1997 Dec;23(6):1094-7. doi: 10.2144/97236st07.