重组痘苗病毒的产生
Generation of Recombinant Vaccinia Viruses.
作者信息
Wyatt Linda S, Earl Patricia L, Moss Bernard
机构信息
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
出版信息
Curr Protoc Protein Sci. 2017 Aug 1;89:5.13.1-5.13.18. doi: 10.1002/cpps.33.
Abstract
This unit describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector or PCR fragment to generate a recombinant virus. Selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses are described. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented. © 2017 by John Wiley & Sons, Inc.
摘要
本单元描述了如何用痘苗病毒感染细胞，然后用质粒转移载体或PCR片段转染细胞以产生重组病毒。还描述了用于分离重组病毒的选择和筛选方法以及重组病毒的扩增方法。最后，介绍了一种主要用于检测重组安卡拉痘苗病毒（MVA）的活细胞免疫染色方法。©2017约翰威立父子公司。
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本文引用的文献
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