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重组痘苗病毒的产生

Generation of Recombinant Vaccinia Viruses.

作者信息

Wyatt Linda S, Earl Patricia L, Moss Bernard

机构信息

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.

出版信息

Curr Protoc Mol Biol. 2017 Jan 5;117:16.17.1-16.17.18. doi: 10.1002/cpmb.32.

DOI:10.1002/cpmb.32
PMID:28060405
Abstract

This unit describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector or PCR fragment to generate a recombinant virus. Selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses are described. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented. © 2017 by John Wiley & Sons, Inc.

摘要

本单元描述了如何用痘苗病毒感染细胞，然后用质粒转移载体或PCR片段转染细胞以产生重组病毒。介绍了用于分离重组病毒的选择和筛选方法以及重组病毒的扩增方法。最后，介绍了一种主要用于检测重组安卡拉痘苗病毒（MVA）的活细胞免疫染色方法。© 2017约翰威立父子公司。

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Generation of Recombinant Vaccinia Viruses.重组痘苗病毒的产生
Curr Protoc Mol Biol. 2017 Jan 5;117:16.17.1-16.17.18. doi: 10.1002/cpmb.32.
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Generation of Recombinant Vaccinia Viruses.重组痘苗病毒的产生
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Generation of recombinant vaccinia viruses.重组痘苗病毒的产生。
Curr Protoc Protein Sci. 2001 May;Chapter 5:Unit5.13. doi: 10.1002/0471140864.ps0513s13.
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Generation of recombinant vaccinia viruses.重组痘苗病毒的产生。
Curr Protoc Mol Biol. 2001 May;Chapter 16:Unit16.17. doi: 10.1002/0471142727.mb1617s43.
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Generation of Recombinant Vaccinia Viruses.重组痘苗病毒的产生
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Nonreplicating vaccinia vector efficiently expresses recombinant genes.非复制型痘苗病毒载体能高效表达重组基因。
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10847-51. doi: 10.1073/pnas.89.22.10847.
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Characterization of recombinant vaccinia viruses and their products.重组痘苗病毒及其产物的特性分析。
Curr Protoc Protein Sci. 2001 May;Chapter 5:Unit5.14. doi: 10.1002/0471140864.ps0514s13.
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Construction and evaluation of a new triple-gene expression cassette vaccinia virus shuttle vector.构建与评价一种新型三重基因表达盒痘苗病毒穿梭载体
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[A novel double expression shuttle vector to get marker-free recombinant modified vaccinia virus Ankara].一种用于获得无标记重组改良痘苗病毒安卡拉的新型双表达穿梭载体
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A recombinant vector derived from the host range-restricted and highly attenuated MVA strain of vaccinia virus stimulates protective immunity in mice to influenza virus.源自宿主范围受限且高度减毒的痘苗病毒MVA株的重组载体可刺激小鼠对流感病毒产生保护性免疫。
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