Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
Department of Applied Chemistry, University of Hyogo 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
Colloids Surf B Biointerfaces. 2017 Oct 1;158:658-666. doi: 10.1016/j.colsurfb.2017.07.042. Epub 2017 Jul 19.
Polymer vesicles formed by a pair of oppositely charged diblock copolyelectrolytes (PICsomes) are considered as a good alternative to polymersomes formed by amphiphilic copolymers. Here, we report on inherent stability and in vitro biocompatibility of PICsomes prepared from a pair of oppositely charged zwitterionic-ionic copolymers, in which the ionic block is a strong polyelectrolyte. Our results demonstrated that the PICsomes are highly stable over a wide range of pH and temperatures. Direct microscopic observations revealed that the PICsomes retain their morphology in the presence of human serum. In vitro studies using human skin fibroblasts (HSFs) showed that the polymer vesicles are not cytotoxic and do not affect cell proliferation and adhesion. A model hydrophilic dye was effectively incorporated into the PICsomes by simple mixing. Using confocal microscopy observations, we demonstrated that the dye-loaded PICsomes are efficiently internalized by the cells and are located predominantly in endo/lysosomal compartments. Thus, the PICsomes have promising potential for use as nanocontainers for substances of biomedical interest.
由一对带相反电荷的两亲嵌段共聚物电解质（PICsomes）形成的聚合物囊泡被认为是由两亲性共聚物形成的聚合物囊泡的良好替代品。在这里，我们报告了由一对带相反电荷的两性离子-离子嵌段共聚物制备的 PICsomes 的固有稳定性和体外生物相容性，其中离子嵌段是一种强聚电解质。我们的结果表明，PICsomes 在很宽的 pH 和温度范围内都具有很高的稳定性。直接的微观观察表明，PICsomes 在存在人血清的情况下保持其形态。体外研究使用人皮肤成纤维细胞（HSFs）表明，聚合物囊泡无细胞毒性，不影响细胞增殖和黏附。一种模型亲水性染料通过简单混合可有效掺入 PICsomes 中。使用共聚焦显微镜观察，我们证明了负载染料的 PICsomes 被细胞有效内化，并主要位于内体/溶酶体隔室中。因此，PICsomes 有望用作生物医学相关物质的纳米容器。
