Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, Brazil.
Instituto Federal de Educação Ciência e Tecnologia de Goiás, Inhumas, Brazil.
Food Chem. 2017 Dec 15;237:1118-1123. doi: 10.1016/j.foodchem.2017.06.010. Epub 2017 Jun 3.
Honey is a functional food widely consumed. Thus, the evaluation of honey samples to determine its phenolic content and antioxidant capacity (AOC) is relevant to determine its quality. Usually AOC is performed by spectrophotometric methods, which lacks reproducibility and practicality. In this context, the electroanalytical methods offer higher simplicity and accuracy. Hence, the aim of this work was to use of electroanalytical tools and laccase based biosensor on the evaluation of AOC and total phenol content (TPC) of honey samples from different countries. The antioxidant power established by electrochemical index presented good correlation with the spectrophotometric FRAP (Ferric Reducing Ability of Plasma) and DPPH (2,2-Diphenyl-1-Picrylhydrazyl) radical scavenging assays. Also, TPC results obtained by the biosensor agreed with the Folin-Ciocalteu (FC) assay. In addition to the semi quantitative results, the electroanalysis offered qualitative parameters, which were useful to indicate the nature of major phenolic compounds.
蜂蜜是一种广泛食用的功能性食品。因此，评估蜂蜜样品以确定其酚类含量和抗氧化能力（AOC）对于确定其质量至关重要。通常，AOC 通过分光光度法进行，但其重现性和实用性较差。在这种情况下，电化学方法提供了更高的简单性和准确性。因此，本工作的目的是使用电化学工具和漆酶生物传感器评估来自不同国家的蜂蜜样品的 AOC 和总酚含量（TPC）。电化学指数确定的抗氧化能力与分光光度法 FRAP（血浆还原能力）和 DPPH（2,2-二苯基-1-苦基肼）自由基清除试验具有良好的相关性。此外，生物传感器获得的 TPC 结果与福林-希卡尔特（FC）测定法一致。除了半定量结果外，电分析还提供了定性参数，这些参数有助于指示主要酚类化合物的性质。
