Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Republic of Korea.
Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea.
Food Chem. 2017 Dec 15;237:191-197. doi: 10.1016/j.foodchem.2017.05.095. Epub 2017 May 18.
This study investigated the effect of atmospheric pressure plasma (APP) treatment on nitrite content and functionality of plant extracts. Ethanolic extracts of Perilla frutescens (EEP) were prepared and treated with APP for 60min. Nitrite content increased from 0 to 45.8mg/l in EEP after APP treatment for 60min. Antimicrobial activity of EEP against Clostridium perfringens and Salmonella Typhimurium was increased by APP with no influence on antioxidative activity (p<0.05). Lyophilized EEP (LEEP) treated with APP for 60min contained 3.74mg/g nitrite. The control (LEEP without APP) contained no nitrite. The minimum inhibitory concentration (MIC) of LEEP for C. perfringens was 200µg/ml. The control did not inhibit C. perfringens growth between 25 and 1000µg/ml. MICs of LEEP and the control against S. Typhimurium were 25 and 50µg/ml, respectively. New nitrite sources with increased antimicrobial activity can be produced from natural plants by APP treatment.
本研究考察了大气压等离子体（APP）处理对亚硝酸盐含量和植物提取物功能的影响。制备了紫苏（EEP）的乙醇提取物，并将其用 APP 处理 60min。经过 60min 的 APP 处理后，EEP 中的亚硝酸盐含量从 0 增加到 45.8mg/l。APP 处理对产气荚膜梭菌和鼠伤寒沙门氏菌的抑菌活性有影响，但对抗氧化活性无影响（p<0.05）。用 APP 处理 60min 的冻干紫苏提取物（LEEP）含有 3.74mg/g 的亚硝酸盐。对照品（未经 APP 处理的 LEEP）不含亚硝酸盐。LEEP 对产气荚膜梭菌的最小抑菌浓度（MIC）为 200µg/ml。对照品在 25 至 1000µg/ml 之间不抑制产气荚膜梭菌的生长。LEEP 和对照品对鼠伤寒沙门氏菌的 MIC 分别为 25 和 50µg/ml。通过 APP 处理可以从天然植物中产生具有增强抗菌活性的新亚硝酸盐来源。
