Morita Mayuko, Naito Yuji, Niki Etsuo, Yoshikawa Toshikazu
Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan; Department of Gastrointestinal Immunology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Food Chem. 2017 Dec 15;237:574-580. doi: 10.1016/j.foodchem.2017.05.157. Epub 2017 May 31.
Unregulated oxidative modification of biological molecules induced by multiple oxidants in vivo has been implicated in the pathogenesis of various diseases. Accordingly, the role of antioxidants contained in foods in the maintenance of health and prevention of diseases has received much attention. The efficacy of antioxidants against oxidative stress depends on the nature of oxidants. In the present study, the antioxidant action of fermented grain food supplement, Antioxidant Biofactor (AOB), for scavenging peroxyl radical and inhibition of plasma lipid oxidation induced by multiple oxidants was measured. The antioxidant efficacy against lipid oxidation was assessed by the level of lipid hydroperoxides produced using diphenyl-1-pyrenylphosphine, which is not fluorescent per se but reacts with lipid hydroperoxides stoichiometrically to yield highly fluorescent diphenyl-1-pyrenylphosphine oxide. AOB acted as a potent peroxyl radical scavenger and suppressed lipid oxidation induced by peroxyl radical, peroxynitrite, hypochlorite, and singlet oxygen, but not by 15-lipoxygenase.
体内多种氧化剂诱导的生物分子无节制氧化修饰与多种疾病的发病机制有关。因此，食物中所含抗氧化剂在维持健康和预防疾病方面的作用备受关注。抗氧化剂对抗氧化应激的功效取决于氧化剂的性质。在本研究中，测定了发酵谷物食品补充剂抗氧化生物因子（AOB）清除过氧自由基以及抑制多种氧化剂诱导的血浆脂质氧化的抗氧化作用。通过使用二苯基-1-芘基膦产生的脂质氢过氧化物水平评估对脂质氧化的抗氧化功效，二苯基-1-芘基膦本身不发荧光，但与脂质氢过氧化物按化学计量反应生成高荧光的二苯基-1-芘基膦氧化物。AOB作为一种有效的过氧自由基清除剂，可抑制过氧自由基、过氧亚硝酸盐、次氯酸盐和单线态氧诱导的脂质氧化，但不能抑制15-脂氧合酶诱导的脂质氧化。
