Liu Jie, Wang Huailing, Liu Xiaoyu, Zhang Guohao, Liu Zhigang
Department of Respirology & Allergy Third Affiliated Hospital of Shenzhen University Shenzhen China.
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University Shenzhen Key Laboratory of Allergy & Immunology Shenzhen University School of Medicine Shenzhen China.
Food Sci Nutr. 2020 May 23;8(7):3164-3172. doi: 10.1002/fsn3.1564. eCollection 2020 Jul.
Chinese liquor is obtained from various grains by fermentation and complex processes. Chinese liquor contains complex ingredients. However, the low contents and presence of ethanol restricted the flavor substances function study. In current study, a flavor substance, homofuraneol (HOMO) was isolated from the Chinese liquor and the potency against HO-induced oxidative damage in HepG2 cells and lifespan-extending ability in were explored. Results indicated that HOMO increased the HepG2 cells cytoactive by eliminating excessive intracellular free radicals, upregulating antioxidant enzyme activity and inhibiting the phosphorylation of mitogen-activated protein kinases (MAPKs) pathway. Further study revealed that HOMO extended the lifespan of N2 nematodes under normal and oxidative stress conditions. Moreover, RT-PCR results showed that paraquat activated the expression of and was significantly regulated by HOMO. Of note, our results indicated that HOMO recovered the redox states of HepG2 cells by targeting MAPKs and upregulating the stress resistance of nematodes by modulating the expression of stress-responsive genes, such as DAF-16.
中国白酒是通过各种谷物经发酵和复杂工艺制成的。中国白酒含有复杂的成分。然而，乙醇含量较低及其存在限制了风味物质功能的研究。在当前研究中，从中国白酒中分离出一种风味物质高呋喃醇（HOMO），并探讨了其对HepG2细胞中HO诱导的氧化损伤的效力以及对线虫寿命延长的能力。结果表明，HOMO通过消除细胞内过多的自由基、上调抗氧化酶活性和抑制丝裂原活化蛋白激酶（MAPKs）途径的磷酸化来提高HepG2细胞的细胞活性。进一步研究表明，HOMO在正常和氧化应激条件下延长了N2线虫的寿命。此外，RT-PCR结果显示百草枯激活了相关基因的表达，且该基因受HOMO的显著调控。值得注意的是，我们的结果表明，HOMO通过靶向MAPKs恢复HepG2细胞的氧化还原状态，并通过调节应激反应基因（如DAF-16）的表达上调线虫的抗逆性。
