Li Yinping, Li Haifei, Wang Shiyao, Li Jing, Bacha Syed Asim Shah, Xu Guofeng, Li Jing
Laboratory of Quality and Safety Risk Assessment for Fruit, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning, China.
Department of Applied Biosciences, Toyo University, Ora-gun, Japan.
Front Plant Sci. 2023 Apr 20;14:1082245. doi: 10.3389/fpls.2023.1082245. eCollection 2023.
As a highly economic small fruit crop, blueberry is enjoyed by most people in terms of color, taste, and rich nutrition. To better understand its coloring mechanism on the process of ripening, an integrative analysis of the metabolome and transcriptome profiles was performed in three blueberry varieties at three developmental stages. In this study, 41 flavonoid metabolites closely related to the coloring in blueberry samples were analyzed. It turned out that the most differential metabolites in the ripening processes were delphinidin-3--arabinoside (dpara), peonidin-3--glucoside (pnglu), and delphinidin-3--galactoside (dpgal), while the most differential metabolites among different varieties were flavonols. Furthermore, to obtain more accurate and comprehensive transcripts of blueberry during the developmental stages, PacBio and Illumina sequencing technology were combined to obtain the transcriptome of the blueberry variety Misty, for the very first time. Finally, by applying the gene coexpression network analysis, the darkviolet and bisque4 modules related to flavonoid synthesis were determined, and the key genes related to two flavonoid 3', 5'-hydroxylase () genes in the darkviolet module and one transcription factor in the bisque4 module were predicted. It is believed that our findings could provide valuable information for the future study on the molecular mechanism of flavonoid metabolites and flavonoid synthesis pathways in blueberries.
蓝莓作为一种经济价值高的小浆果作物，其色泽、口感和丰富的营养深受大众喜爱。为了更好地了解其成熟过程中的着色机制，对三个蓝莓品种在三个发育阶段的代谢组和转录组图谱进行了综合分析。本研究分析了41种与蓝莓样本着色密切相关的类黄酮代谢物。结果表明，成熟过程中差异最大的代谢物是矢车菊素 - 3 - 阿拉伯糖苷（dpara）、芍药素 - 3 - 葡萄糖苷（pnglu）和矢车菊素 - 3 - 半乳糖苷（dpgal），而不同品种间差异最大的代谢物是黄酮醇。此外，为了获得蓝莓发育阶段更准确、全面的转录本，首次将PacBio和Illumina测序技术相结合，获得了蓝莓品种密斯提的转录组。最后，通过应用基因共表达网络分析，确定了与类黄酮合成相关的深紫色和米黄色4模块，并预测了深紫色模块中与两个类黄酮3'，5'-羟化酶（）基因和米黄色4模块中一个转录因子相关的关键基因。相信我们的研究结果可为今后蓝莓类黄酮代谢物分子机制及类黄酮合成途径的研究提供有价值的信息。
