西班牙血红色和Verdal仙人掌（仙人掌属，印度无花果种）组织类胡萝卜素谱的表征
Characterization of carotenoid profile of Spanish Sanguinos and Verdal prickly pear (Opuntia ficus-indica, spp.) tissues.
作者信息
Cano M P, Gómez-Maqueo A, García-Cayuela T, Welti-Chanes J
机构信息
Centro de Biotecnología FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Av. Eugenio Garza Sada 2501 Sur, Col. Tecnológico, Monterrey, NL, Mexico; Laboratory of Phytochemistry and Plant Food Functionality, Biotechnology and Food Microbiology Department, Institute of Food Science Research (CIAL) (CSIC-UAM), C/ Nicolás Cabrera, 9, 28049 Madrid, Spain.
Centro de Biotecnología FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Av. Eugenio Garza Sada 2501 Sur, Col. Tecnológico, Monterrey, NL, Mexico.
出版信息
Food Chem. 2017 Dec 15;237:612-622. doi: 10.1016/j.foodchem.2017.05.135. Epub 2017 May 29.
Carotenoid profiles of different tissues (peel, pulp and whole fruit) of Spanish Sanguinos (red) and Verdal (orange) prickly pears (Opuntia ficus-indica spp.) have been characterized in detail and quantified for the first time. Carotenoids were determined by HPLC-PDA-MS (APCI), using a reverse phase C column. A total of 9 xantophylls and 4 hydrocarbon carotenes were identified. Also, minor amounts of chlorophyll a, a' and b can be observed in Opuntia peel extracts. All carotenoids were found to be present in their free form (no carotenoid esters were detected). The RAE was highest in Opuntia peels, showing values from 19.20 to 16.48µg/100g fresh weigth, for Sanguinos and Verdal Opuntia fruits, respectively. The main carotenoid in Opuntia peel extracts was (all-E)-lutein with 1132.51 and 767.98µg/100g fresh weigth, followed by (all-E)-β-carotene with 200.40 and 173.50µg/100g fresh weigth for Sanguinos and Verdal varieties of Opuntia fruits, respectively.
首次对西班牙血红色（红色）和弗尔达尔（橙色）仙人掌果（仙人掌属梨果仙人掌种）不同组织（果皮、果肉和整个果实）中的类胡萝卜素谱进行了详细表征和定量分析。使用反相C柱，通过HPLC-PDA-MS（大气压化学电离）测定类胡萝卜素。共鉴定出9种叶黄素和4种烃类胡萝卜素。此外，在仙人掌果皮提取物中可观察到少量叶绿素a、a'和b。所有类胡萝卜素均以游离形式存在（未检测到类胡萝卜素酯）。仙人掌果皮中的视黄醇活性当量（RAE）最高，血红色和弗尔达尔仙人掌果的含量分别为19.20至16.48µg/100g鲜重。仙人掌果皮提取物中的主要类胡萝卜素是（全反式）叶黄素，血红色和弗尔达尔品种仙人掌果的含量分别为1132.51和767.98µg/100g鲜重，其次是（全反式）β-胡萝卜素，含量分别为200.40和173.50µg/100g鲜重。
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