Makkar Hardik, Patri Gaurav
Postgraduate student, Department of Conservative Dentistry and Endodontics, Kalinga Institute of Dental Sciences, Bhubaneswar, Odisha, India.
Professor, Department of Conservative Dentistry and Endodontics, Kalinga Institute of Dental Sciences, Bhubaneswar, Odisha, India.
J Clin Diagn Res. 2017 Jun;11(6):ZC05-ZC08. doi: 10.7860/JCDR/2017/27633.9957. Epub 2017 Jun 1.
Nanotechnology is gaining momentum in the search for ideal biomaterials by dental researchers. The expansible nature of Endodontology deems exploration, learning and scrutinizing newer avenues which have the potential to be applied and investigated. The popularity of polymers as drug delivery vehicles has opened avenues for their application in the root canal system. This study utilized application of biodegradable polymers as potential drug delivery vehicles against , one of the main reasons for post treatment disease.
This study aimed at fabricating Poly (Lactic-co-Glycolic Acid) (PLGA)-moxifloxacin nanoparticles and assessing its sustained antimicrobial efficacy with calcium hydroxide and chitosan-moxifloxacin hydrogel against .
PLGA (50:50) in a quantity of 100 mg was dissolved in 1 ml ethyl acetate and 45 ml of 0.3% w/v Vitamin-E Polyethylene Glycol 1000 Succinate (vitamin E-TPGS) was kept for magnetic stirring in separate beaker. Moxifloxacin (50 μl) was added to polymer PLGA following which vitamin E-TPGS was added to the polymer. Nanoparticles were fabricated using ultrasonication and collected by centrifugation. Surface characterization was assessed using scanning electron microscope. Results were obtained in the form of zone of inhibition by the nanoparticles against and comparisons were made with chitosan-moxifloxacin hydrogel and calcium hydroxide using Analysis of Variance (ANOVA) followed by Student t-test.
Upon statistical analysis, the zone of inhibition against . remained constant with PLGA-moxifloxacin nanoparticles for 14 days while it decreased with chitosan-moxifloxacin hydrogel and remained nil for calcium hydroxide (p<0.01).
The study posits that PLGA encapsulated moxifloxacin nanoparticles showcased sustained antibacterial effect in low doses against the test pathogen. Its sustained and programmed release makes them unique contenders for further evaluation in Endodontics as potential intracanal medicaments.
在寻找理想生物材料的过程中，纳米技术正日益受到牙科研究人员的关注。牙髓病学的不断发展促使人们探索、学习和审视具有应用和研究潜力的新途径。聚合物作为药物递送载体的普及为其在根管系统中的应用开辟了道路。本研究利用可生物降解聚合物作为潜在的药物递送载体，以对抗治疗后疾病的主要原因之一。
本研究旨在制备聚（乳酸-乙醇酸共聚物）（PLGA）-莫西沙星纳米颗粒，并评估其与氢氧化钙和壳聚糖-莫西沙星水凝胶相比对……的持续抗菌效果。
将100mg的PLGA（50:50）溶解于1ml乙酸乙酯中，45ml的0.3%w/v维生素E聚乙二醇1000琥珀酸酯（维生素E-TPGS）置于单独的烧杯中进行磁力搅拌。将50μl莫西沙星加入到聚合物PLGA中，随后将维生素E-TPGS加入到聚合物中。通过超声处理制备纳米颗粒，并通过离心收集。使用扫描电子显微镜评估表面特征。以纳米颗粒对……的抑菌圈形式获得结果，并使用方差分析（ANOVA）随后进行学生t检验，与壳聚糖-莫西沙星水凝胶和氢氧化钙进行比较。
经统计分析，PLGA-莫西沙星纳米颗粒对……的抑菌圈在14天内保持恒定，而壳聚糖-莫西沙星水凝胶的抑菌圈减小，氢氧化钙的抑菌圈为零（p<0.01）。
该研究表明，PLGA包裹的莫西沙星纳米颗粒在低剂量下对测试病原体展现出持续的抗菌效果。其持续和程序性释放使其成为牙髓病学中作为潜在根管内药物进行进一步评估的独特候选者。
