利用 Illumina 测序技术探索健康和患病种植体周围位点的微生物组。

Exploring the microbiome of healthy and diseased peri-implant sites using Illumina sequencing.

机构信息

Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.

Department of Dental Ecology, University of North Carolina at Chapel Hill School of Dentistry, Chapel Hill, NC, USA.

出版信息

J Clin Periodontol. 2017 Dec;44(12):1274-1284. doi: 10.1111/jcpe.12788. Epub 2017 Nov 21.

DOI:10.1111/jcpe.12788

PMID:28766745

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7209775/

Abstract

AIM

To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome.

MATERIALS AND METHODS

Submucosal biofilms from 32 H and 35 P sites were analysed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using principal coordinate analysis (PCoA), t tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined.

RESULTS

PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p < .05). Porphyromonas and Treponema were more abundant in P while Rothia and Neisseria were higher in H (p < .05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola, and P. gingivalis levels were higher in P, as well as F. alocis, F. fastidiosum and T. maltophilum (p < .05).

CONCLUSION

The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harbouring traditional and new pathogens. The core peri-implant microbiome harbours taxa from genera often associated with periodontal inflammation.

摘要

目的

比较健康（H）和患病（P）种植体周围部位的微生物组，确定核心种植体周围微生物组。

材料和方法

使用 16S rRNA 测序（MiSeq，Illumina）、QIIME 和 HOMINGS 分析来自 32 个 H 和 35 个 P 部位的黏膜下生物膜。使用主坐标分析（PCoA）、t 检验和 Wilcoxon 秩和检验以及 FDR 调整来确定组间差异。确定种植体周围核心微生物组。

结果

PCoA 在所有分类水平上均显示 H 和 P 之间的分区。拟杆菌门、螺旋体门和共生菌门在 P 中含量较高，而放线菌门在 H 中占优势（p<.05）。卟啉单胞菌属和密螺旋体属在 P 中更为丰富，而罗氏菌属和奈瑟菌属在 H 中含量较高（p<.05）。核心种植体周围微生物组包含梭杆菌属、卟啉单胞菌属和 Campylobacter sp. T. denticola，以及 P. gingivalis 在 P 中的水平较高，还有 F. alocis、F. fastidiosum 和 T. maltophilum（p<.05）。