Fenske M
Comp Biochem Physiol A Comp Physiol. 1986;85(2):263-71. doi: 10.1016/0300-9629(86)90249-5.
In the absence of HCG, production of testosterone by whole testes superfused in vitro was quite constant during the 5-hr superfusion period. Addition of 23-184 mIU/ml HCG caused a significant increase of testosterone production which was apparent from 30 min after start of superfusion. Basal and HCG-stimulated testosterone production by whole testes was significantly higher (400, 1950 ng/testis/5 hr, without and with 100 mIU HCG) than by isolated cells (200, 1350 ng/testis/5 hr). Incubation of isolated interstitial cells in medium 199 supplemented with fetal calf serum (FCS), (N-2-hydroxyethylpiperazine-N-2-ethanesulphonic acid, HEPES) and 3-isobutyl-methylxanthine (MIX), and in medium 199 without FCS, HEPES or MIX, gave similar testosterone responses. While centrifugation at 8000 g for 2 min drastically diminished testosterone formation by isolated interstitial cells, production was similar by cells incubated in either 0.5, 1.0 or 1.5 ml medium. A significant decrease of testosterone synthesis by isolated interstitial cells was found when cells were stored at 4 degrees C for 2 days and then were incubated at 35 degrees C for 6 hr without or with 1-1000 microIU HCG. While isolated interstitial cells incubated at 5 degrees C did not produce testosterone at all, testosterone production increased to 49.5 +/- 3.9 ng/10(5) cells (30 degrees C) and 24.1 +/- 1.1 ng/10(5) cells (40 degrees C), respectively. HCG-stimulated testosterone production was maximal when interstitial cells were incubated at 34 degrees C.
在缺乏人绒毛膜促性腺激素（HCG）的情况下，体外灌流的整个睾丸在5小时灌流期内睾酮的产生相当恒定。添加23 - 184 mIU/ml的HCG会导致睾酮产生显著增加，从灌流开始后30分钟就很明显。整个睾丸的基础睾酮产生量和HCG刺激的睾酮产生量（无HCG时为400 ng/睾丸/5小时，有100 mIU HCG时为1950 ng/睾丸/5小时）显著高于分离细胞（无HCG时为200 ng/睾丸/5小时，有HCG时为1350 ng/睾丸/5小时）。在补充有胎牛血清（FCS）、（N - 2 - 羟乙基哌嗪 - N' - 2 - 乙磺酸，HEPES）和3 - 异丁基 - 甲基黄嘌呤（MIX）的199培养基中以及在不含FCS、HEPES或MIX的199培养基中培养分离的间质细胞，得到相似的睾酮反应。虽然以8000 g离心2分钟会显著减少分离的间质细胞的睾酮形成，但在0.5、1.0或1.5 ml培养基中培养的细胞产生量相似。当分离的间质细胞在4℃储存2天，然后在35℃培养6小时，无论有无1 - 1000微国际单位的HCG，都发现睾酮合成显著减少。虽然在5℃培养的分离间质细胞根本不产生睾酮，但睾酮产生量分别增加到49.5±3.9 ng/10⁵细胞（30℃）和24.1±1.1 ng/10⁵细胞（40℃）。当间质细胞在34℃培养时，HCG刺激的睾酮产生量最大。
