Woessner J P, Gillham N W, Boynton J E
Gene. 1986;44(1):17-28. doi: 10.1016/0378-1119(86)90038-7.
The chloroplast (cp)-encoded CF1 ATPase beta-subunit gene (atpB) of Chlamydomonas reinhardtii and its flanking regions have been sequenced. The derived amino acid (aa) sequence is highly homologous to that of the beta-subunit gene in Escherichia coli, bovine heart mitochondria, and higher plant cp. In contrast to all other cp genomes, the CF1 epsilon subunit gene (atpE) does not lie at the 3' end of the atpB gene but maps to a position 92 kb away in the other single-copy region. Northern blots confirm that the beta subunit is not encoded as part of a dicistronic message as it is in higher plants. The region just upstream from the atpB gene in C. reinhardtii contains two small open reading frames (ORFs) and not the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase as is found in cp genomes of higher plants. No transcripts for either ORF were detected, but the codon usage in these ORFs as well as in the atpB gene follows the unique pattern of codon usage previously seen in other cp genes in C. reinhardtii.
莱茵衣藻叶绿体（cp）编码的CF1 ATP酶β亚基基因（atpB）及其侧翼区域已被测序。推导的氨基酸（aa）序列与大肠杆菌、牛心线粒体和高等植物叶绿体中的β亚基基因高度同源。与所有其他叶绿体基因组不同，CF1ε亚基基因（atpE）并不位于atpB基因的3'端，而是定位于另一个单拷贝区域中距离其92 kb的位置。Northern杂交证实，β亚基不像在高等植物中那样作为双顺反子信息的一部分进行编码。莱茵衣藻atpB基因上游区域包含两个小的开放阅读框（ORF），而不像高等植物叶绿体基因组中那样含有1,5-二磷酸核酮糖羧化酶/加氧酶大亚基的基因。未检测到任何一个ORF的转录本，但这些ORF以及atpB基因中的密码子使用遵循了莱茵衣藻其他叶绿体基因中先前发现的独特密码子使用模式。
