Rao Mkk, Kapila K, Gupta R M
Readers, Department of Microbiology, Armed Forces Medical College, Pune - 411040.
Med J Armed Forces India. 1997 Jan;53(1):45-51. doi: 10.1016/S0377-1237(17)30644-5. Epub 2017 Jun 26.
Enzyme linked immunosorbent assay (ELISA) has now gained wide acclaim in the immunodiagnosis of infectious diseases. ELISA is continuously evolving with newer and sensitive formulations being added to it. The basic tenets of ELISA are discussed. The role of ELISA in two important issues facing us today, namely tuberculosis and human immunodeficiency virus infection are reviewed.
酶联免疫吸附测定(ELISA)现已在传染病免疫诊断中广受赞誉。ELISA不断发展,新的、更灵敏的检测方法不断涌现。本文讨论了ELISA的基本原理。综述了ELISA在当今我们面临的两个重要问题,即结核病和人类免疫缺陷病毒感染中的作用。