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细胞悬液酶免疫测定:血清抗体与细胞表面抗原反应的研究

Enzyme immunoassay with cell suspensions: studies on reactions between serum antibodies and cell-surface antigens.

作者信息

Wasik M, Muirhead D, Rubocki R, Milgrom F

出版信息

Int Arch Allergy Appl Immunol. 1986;81(3):269-75. doi: 10.1159/000234146.

DOI:10.1159/000234146
PMID:2876966
Abstract

The following reactions of serum antibodies with cell surface antigens were studied by means of an enzyme immunoassay with cell suspensions: Rh antiserum with human red blood cells; H-2 antisera with murine red blood cells; H-2 antisera with murine thymocytes and hepatocytes; Thy-1 antiserum with murine thymocytes; polyvalent and monovalent HLA alloantisera with human leukocytes and human mononuclear cells, and antisera of murine origin with human and marmoset lymphoid cell lines. In all instances, with the exception of monovalent HLA antisera, the assay proved to be a sensitive and highly specific procedure.

摘要

通过细胞悬液酶免疫测定法研究了血清抗体与细胞表面抗原的以下反应

用人类红细胞检测Rh抗血清;用小鼠红细胞检测H-2抗血清;用小鼠胸腺细胞和肝细胞检测H-2抗血清;用小鼠胸腺细胞检测Thy-1抗血清;用人类白细胞和人类单核细胞检测多价和单价HLA同种异体抗血清,以及用人类和狨猴淋巴样细胞系检测鼠源抗血清。在所有情况下,除单价HLA抗血清外,该测定法被证明是一种灵敏且高度特异的方法。

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Enzyme immunoassay with cell suspensions: studies on reactions between serum antibodies and cell-surface antigens.细胞悬液酶免疫测定:血清抗体与细胞表面抗原反应的研究
Int Arch Allergy Appl Immunol. 1986;81(3):269-75. doi: 10.1159/000234146.
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Specific lysis of murine cells expressing HLA molecules by allospecific human and murine H-2-restricted anti-HLA T killer lymphocytes.同种特异性人和鼠的H-2限制性抗HLA T杀伤淋巴细胞对表达HLA分子的鼠细胞的特异性裂解作用。
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