Parr E L
Transplantation. 1979 Jan;27(1):45-8. doi: 10.1097/00007890-197901000-00012.
Antigens of the mouse H-2 locus were studied on isolated liver cells by immunoferritin labeling. Cell suspensions were obtained by gentle homogenization of mouse livers that had been perfused with hypertonic sucrose to loosen cell junctions. Hepatocytes showed sparse labeling of H-2 antigens on the order of 1% of that present on peritoneal monocytes. The slight hepatocyte labeling appeared to be specific for the H-2 locus, since there was essentially no label on congenic control hepatocytes. Other cells in the liver cell preparations were more heavily labeled. Bile duct epithelial cells showed heavy labeling on their lateral surface membranes, but none on their apical brush borders. The preparations contained another nonparenchymal cell type that was densely labeled, but it was not positively identified. Spleen lymphocytes added to liver homogenates and carried through the cell isolation procedure with liver cells showed typically heavy ferritin labeling. These observations suggest that the expression of histocompatibility antigens by tissue cells is likely to be quite variable.
通过免疫铁蛋白标记法对分离出的肝细胞上的小鼠H-2基因座抗原进行了研究。通过用高渗蔗糖灌注小鼠肝脏以松开细胞连接，然后轻柔匀浆获得细胞悬液。肝细胞显示出H-2抗原的稀疏标记，约为腹膜单核细胞上H-2抗原的1%。轻微的肝细胞标记似乎对H-2基因座具有特异性，因为同基因对照肝细胞上基本没有标记。肝细胞制剂中的其他细胞标记更重。胆管上皮细胞在其侧表面膜上显示出重标记，但在其顶端刷状缘上没有标记。制剂中含有另一种被密集标记的非实质细胞类型，但未得到确切鉴定。添加到肝脏匀浆中并与肝细胞一起经过细胞分离程序的脾淋巴细胞显示出典型的重铁蛋白标记。这些观察结果表明，组织细胞对组织相容性抗原的表达可能有很大差异。
